Int J Med Sci 2018; 15(13):1517-1521. doi:10.7150/ijms.28337
Ethanol Extract from Brucea Javanica Seed Inhibits Angiogenesis Mediated by Platelet Derived Growth Factor Receptor-beta
1. Key Laboratory of Pathobiology, Ministry of Education, Norman Bethune College of Medicine, Jilin University, Changchun, China
2. People's Hospital of Jilin Province and Changchun University of Chinese Medicine, Changchun, P.R. China
3. Second Hospital of Jilin University, Changchun, China
4. Current address: Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences, Tianjin, China
#These authors contributed equally to this work.
Wang X, Li Y, Mou Y, Yue Z, Zhang H, Li R, Sun H. Ethanol Extract from Brucea Javanica Seed Inhibits Angiogenesis Mediated by Platelet Derived Growth Factor Receptor-beta. Int J Med Sci 2018; 15(13):1517-1521. doi:10.7150/ijms.28337. Available from http://www.medsci.org/v15p1517.htm
The present study aimed to investigate the effects of ethanol extract from Brucea javanicaseed (EEBJS) on the angiogenesis of human umbilical vein endothelial cells (HUVECs) and the possible molecular signal involved. Firstly, a Matrigel-based in vitro angiogenesis assay demonstrated that EEBJS inhibited the angiogenesis of HUVECs in a dose-dependent manner. Then by using porcine aortic endothelial cells which stably express human PDGFR-beta, we found that the inhibition of angiogenesis was mediated by PDGFR-beta. Taken together, we conclude that EEBJS inhibited the angiogenesis function of the vascular endothelial cells mediated by PDGFR-beta, and postulate that it might contribute to the therapeutic effects of EEBJS on malignant tumors.
Keywords: Ethanol Extract from Brucea javanica Seed, Angiogenesis, Human Umbilical Vein Endothelial Cells, Platelet Derived Growth Factor Receptor, Malignant Tumors