1. Department of Microbiology and Immunology, Kunming Medical University, Kunming 650500, China, 2. Department of Biochemistry and Molecular Biology, Kunming Medical University, Kunming 650500, China, 3. Yunnan Province Key Laboratory for Tropical Infectious Diseases in Universities, Kunming Medical University, Kunming 650500, China, 4. The Institute for Tropical Medicine, Kunming Medical University, Kunming 650500, China, 5. Yunnan Demonstration Base of International Science and Technology Cooperation for Tropical Diseases, Kunming 650500, China, 6. Yunnan Province Integrative Innovation Center for Public Health, Diseases Prevention and Control, Kunming Medical University, Kunming 650500, China. *These authors contributed equally to the study.
✉ Corresponding authors: E-mail: AihuaLiu, liuaihuaedu.cn; FukaiBao, baofukaiedu.cn; Tel: 86-13888369882; Fax: 86-871-65922857; Address: Kunming Medical University, 1186 Chunrongxi road, Chenggong District, Kunming 650500, Yunnan, P. R. of China.More
Citation:
Peng Y, Liang Z, Liu A, Li E, Dai X, Bai R, Ji Z, Jian M, Ma M, Tao L, Bao F, Wang F, Bi Y, Ding Z, Manzama-Esso A. Borrelia burgdorferi basic membrane protein A stimulates murine macrophage to secrete specific chemokines. Int J Med Sci 2018; 15(13):1473-1479. doi:10.7150/ijms.26657. https://www.medsci.org/v15p1473.htm
In this study, we investigated the mechanisms that lead to the production of proinflammatory mediators by the murine macrophage cell line, RAW264.7, when these cells are exposed in vitro to recombinant Borrelia burgdorferi basic membrane protein A (rBmpA). Using antibody protein microarray technology with high-throughput detection ability for detecting 25 chemokines in culture supernatant the RAW264.7 cell culture supernatants at 12 and 24 h post-stimulation with rBmpA, we identified two chemokines, a monocyte chemoattractant protein-5 (MCP-5/CCL12) and a macrophage inflammatory protein-2 (MIP-2/CXCL2), both of which increased significantly after stimulation. We then chose these two chemokines for further study. Enzyme-linked immunosorbent assay and real-time polymerase chain reaction revealed that with the increase of rBmpA concentration, MCP-5/CCL12 and MIP-2/CXCL2 showed concentration-dependent increases (p <0.01).Our results indicate that the rBmpA could stimulate the secretion of several specific chemokines and induce Lyme arthritis.
Keywords: Borrelia burgdorferi, basic membrane protein A (rBmpA), murine macrophage cell line, RAW264.7, chemokine, Lyme arthritis
Citation styles
APA
Peng, Y., Liang, Z., Liu, A., Li, E., Dai, X., Bai, R., Ji, Z., Jian, M., Ma, M., Tao, L., Bao, F., Wang, F., Bi, Y., Ding, Z., Manzama-Esso, A. (2018). Borrelia burgdorferi basic membrane protein A stimulates murine macrophage to secrete specific chemokines. International Journal of Medical Sciences, 15(13), 1473-1479. https://doi.org/10.7150/ijms.26657.
ACS
Peng, Y.; Liang, Z.; Liu, A.; Li, E.; Dai, X.; Bai, R.; Ji, Z.; Jian, M.; Ma, M.; Tao, L.; Bao, F.; Wang, F.; Bi, Y.; Ding, Z.; Manzama-Esso, A. Borrelia burgdorferi basic membrane protein A stimulates murine macrophage to secrete specific chemokines. Int. J. Med. Sci. 2018, 15 (13), 1473-1479. DOI: 10.7150/ijms.26657.
NLM
Peng Y, Liang Z, Liu A, Li E, Dai X, Bai R, Ji Z, Jian M, Ma M, Tao L, Bao F, Wang F, Bi Y, Ding Z, Manzama-Esso A. Borrelia burgdorferi basic membrane protein A stimulates murine macrophage to secrete specific chemokines. Int J Med Sci 2018; 15(13):1473-1479. doi:10.7150/ijms.26657. https://www.medsci.org/v15p1473.htm
CSE
Peng Y, Liang Z, Liu A, Li E, Dai X, Bai R, Ji Z, Jian M, Ma M, Tao L, Bao F, Wang F, Bi Y, Ding Z, Manzama-Esso A. 2018. Borrelia burgdorferi basic membrane protein A stimulates murine macrophage to secrete specific chemokines. Int J Med Sci. 15(13):1473-1479.
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