Int J Med Sci 2018; 15(12):1349-1354. doi:10.7150/ijms.27609 This issue Cite

Research Paper

GABAergic Alterations in the Rat Testis after Methamphetamine Exposure

Paweena Kaewman1,2, Sutisa Nudmamud-Thanoi1,2, Samur Thanoi1,2✉

1. Department of Anatomy, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand.
2. Centre of Excellence in Medical Biotechnology, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand.

Citation:
Kaewman P, Nudmamud-Thanoi S, Thanoi S. GABAergic Alterations in the Rat Testis after Methamphetamine Exposure. Int J Med Sci 2018; 15(12):1349-1354. doi:10.7150/ijms.27609. https://www.medsci.org/v15p1349.htm
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Abstract

Gamma-aminobutyric acid (GABA), GABA-A receptors and GABA transporter 1 (GAT1) were reported to be involved in the proliferation of Leydig cells, testosterone production and spermatogenesis. Since methamphetamine (METH) has been reported to have adverse effects on testis and its functions, the aim of this study was therefore to determine the changes of GABAergic activity in testis after METH exposure. Male Sprague-Dawley rats were divided into control, acute binge (AB-METH), escalating dose (ED METH) and escalating dose-binge (ED-binge METH) groups. After sacrifice, rat testes were removed and used to estimate GABA concentration and the expression of GABA-A receptor, GAD1, GAD2 and GAT1 genes by using HPLC and RT-PCR, respectively. The GABA concentration was significantly increased in all METH-administrated groups. In addition, significant increases of GABA-A α1 receptor and GAD1 genes expression were found in the ED-binge METH group. Gene expressions of GAT1 were numerically decreased in all METH-administrated rats and reached significant in the ED METH group. These results indicated a compensatory upregulation of GABA production and its functions in testis after METH exposure. Thus, these changes might represent a homeostatic response of GABAergic to the adverse effects of METH.

Keywords: Rat testis, Methamphetamine, GABA, GABA-A α1 receptor, Glutamate decarboxylase, GAT1.


Citation styles

APA
Kaewman, P., Nudmamud-Thanoi, S., Thanoi, S. (2018). GABAergic Alterations in the Rat Testis after Methamphetamine Exposure. International Journal of Medical Sciences, 15(12), 1349-1354. https://doi.org/10.7150/ijms.27609.

ACS
Kaewman, P.; Nudmamud-Thanoi, S.; Thanoi, S. GABAergic Alterations in the Rat Testis after Methamphetamine Exposure. Int. J. Med. Sci. 2018, 15 (12), 1349-1354. DOI: 10.7150/ijms.27609.

NLM
Kaewman P, Nudmamud-Thanoi S, Thanoi S. GABAergic Alterations in the Rat Testis after Methamphetamine Exposure. Int J Med Sci 2018; 15(12):1349-1354. doi:10.7150/ijms.27609. https://www.medsci.org/v15p1349.htm

CSE
Kaewman P, Nudmamud-Thanoi S, Thanoi S. 2018. GABAergic Alterations in the Rat Testis after Methamphetamine Exposure. Int J Med Sci. 15(12):1349-1354.

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