Int J Med Sci 2018; 15(12):1341-1348. doi:10.7150/ijms.27543
Thrombopoietin could protect cerebral tissue against ischemia-reperfusion injury by suppressing NF-κB and MMP-9 expression in rats
1. Department of Neurology, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China
2. Department of Neurology, The First Affiliated Hospital of Henan University of Science and Technology
3. National Clinical Research Center for Mental Disorders, the Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China
Wenjuan Wu and Wei Zhong contributed equally to this work
Wu W, Zhong W, Lang B, Hu Z, He J, Tang X. Thrombopoietin could protect cerebral tissue against ischemia-reperfusion injury by suppressing NF-κB and MMP-9 expression in rats. Int J Med Sci 2018; 15(12):1341-1348. doi:10.7150/ijms.27543. Available from http://www.medsci.org/v15p1341.htm
Objective: To determine the neuroprotective effects and underpinning mechanisms of thrombopoietin (TPO), Matrix Metalloproteinase-9(MMP-9) and Nuclear Factor-κB (NF-κB) after focal cerebral ischemia-reperfusion in rats.
Methods: Male rats underwent 2 hours of right middle cerebral artery occlusion (MCAO) followed by 22 hours of reperfusion. PBS or TPO (0.1ug/kg) was administered from caudal vein before reperfusion. Neurologic deficits, brain edema, Evans blue (EB) extravasation, NF-κB and MMP-9 expression were subsequently examined.
Results: Ischemia-reperfusion injury produced a large area of edema. TPO significantly reduced edema and alleviated neurologic deficits after ischemia-reperfusion. Ischemia-induced increases of NF-κB, MMP-9 and Evans blue extravasation were reduced by TPO intervention.
Conclusion: TPO improved neurological function and ameliorated brain edema after stroke, partly by reducing the ischemia-induced increase of NF-κB and MMP-9.
Keywords: Thrombopoietin (TPO), Nuclear factor-κB (NF-κB), Matrix Metalloproteinase-9 (MMP-9), Ischemia-Reperfusion (IR)