1. Division of Chest Medicine, Department of Internal Medicine, Kaohsiung Armed Forces General Hospital, Kaohsiung City, Taiwan, ROC 2. Institute of Biochemistry, Microbiology and Immunology, Chung Shang Medical University, Taichung City, Taiwan. 3. Clinical Laboratory, Chung Shan Medical University Hospital, Taichung, Taiwan 4. Institute and Department of Food Science, Central Taiwan University of Science and Technology, Taichung, Taiwan.
✉ Corresponding authors: Yih-Shou Hsieh, Ph.D., Institute of Biochemistry, Microbiology and immunology, Chung Shan Medical University, No 110, Section 1, Chien Kuo N. Road, Taichung 402, Taiwan, Tel.: +886-4-2473-0022 ext 11678, E-mail: csmcyshedu.tw and Pei-Ni Chen, Ph.D., Institute of Biochemistry, Microbiology and immunology, Chung Shan Medical University, No 110, Section 1, Chien Kuo N. Road, Taichung 402, Taiwan, Tel.: +886-4-2473-0022 ext 12132, E-mail: peiniedu.twMore
Citation:
Huang SF, Chu SC, Hsieh YH, Chen PN, Hsieh YS. Viola Yedoensis Suppresses Cell Invasion by Targeting the Protease and NF-κB Activities in A549 and Lewis Lung Carcinoma Cells. Int J Med Sci 2018; 15(4):280-290. doi:10.7150/ijms.22793. https://www.medsci.org/v15p0280.htm
Cancer metastasis is a vital trait in malignancies with complicated early diagnosis and therapeutic management. Therefore, the development of new remedies and the utilization of natural medicines that target metastasis are of great interest and have been studied extensively. Chinese medicinal herbs have various anti-carcinogenesis properties; however, the in vitro effect and mechanism of Viola yedoensis on cancer cell metastasis remains poorly understood. V. yedoensis extracts (VYE) can suppress the invasion of a highly metastatic human lung cancer cell line, A549 cells. According to gelatin zymography and casein zymography assays, VYE inhibited the activities of matrix metalloproteinases (MMPs) and urokinase-type plasminogen activator (u-PA). The results of reverse transcription-polymerase chain reaction and Western blotting revealed that VYE can alter the expression of proteinase inhibitor. VYE also suppressed the DNA binding activity of nuclear factor-kappa B. We concluded that VYE may inhibit tumor invasion by suppressing the activities of MMP and u-PA in lung cancer cells.
Huang, S.F., Chu, S.C., Hsieh, Y.H., Chen, P.N., Hsieh, Y.S. (2018). Viola Yedoensis Suppresses Cell Invasion by Targeting the Protease and NF-κB Activities in A549 and Lewis Lung Carcinoma Cells. International Journal of Medical Sciences, 15(4), 280-290. https://doi.org/10.7150/ijms.22793.
ACS
Huang, S.F.; Chu, S.C.; Hsieh, Y.H.; Chen, P.N.; Hsieh, Y.S. Viola Yedoensis Suppresses Cell Invasion by Targeting the Protease and NF-κB Activities in A549 and Lewis Lung Carcinoma Cells. Int. J. Med. Sci. 2018, 15 (4), 280-290. DOI: 10.7150/ijms.22793.
NLM
Huang SF, Chu SC, Hsieh YH, Chen PN, Hsieh YS. Viola Yedoensis Suppresses Cell Invasion by Targeting the Protease and NF-κB Activities in A549 and Lewis Lung Carcinoma Cells. Int J Med Sci 2018; 15(4):280-290. doi:10.7150/ijms.22793. https://www.medsci.org/v15p0280.htm
CSE
Huang SF, Chu SC, Hsieh YH, Chen PN, Hsieh YS. 2018. Viola Yedoensis Suppresses Cell Invasion by Targeting the Protease and NF-κB Activities in A549 and Lewis Lung Carcinoma Cells. Int J Med Sci. 15(4):280-290.
This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions.