Mitochondrial stress and redox failure in steroid-associated osteonecrosis

Tsuchiya, Masanobu; Ichiseki, Toru; Ueda, Shusuke; Ueda, Yoshimichi; Shimazaki, Miyako; Kaneuji, Ayumi; Kawahara, Norio

doi:10.7150/ijms.22525



Theranostics

International Journal of Biological Sciences

Nanotheranostics

Journal of Cancer

Journal of Genomics

Global reach, higher impact

Full Text | PDF

Int J Med Sci 2018; 15(3):205-209. doi:10.7150/ijms.22525 This issue Cite

Research Paper

Mitochondrial stress and redox failure in steroid-associated osteonecrosis

Masanobu Tsuchiya¹, Toru Ichiseki^1✉, Shusuke Ueda¹, Yoshimichi Ueda², Miyako Shimazaki², Ayumi Kaneuji¹, Norio Kawahara¹

1. Department of Orthopaedic Surgery,Kanazawa Medical University, Daigaku 1-1,Uchinada, Kahoku-gun, Ishikawa, 920-0293, Japan
2. Department of Pathology, Kanazawa Medical University, Daigaku 1-1, Uchinada, Kahoku-gun, Ishikawa, 920-0293, Japan

Citation:

Tsuchiya M, Ichiseki T, Ueda S, Ueda Y, Shimazaki M, Kaneuji A, Kawahara N. Mitochondrial stress and redox failure in steroid-associated osteonecrosis. Int J Med Sci 2018; 15(3):205-209. doi:10.7150/ijms.22525. https://www.medsci.org/v15p0205.htm

Other styles

Abstract

The purpose of the role of antioxidant enzymes and mitochondria in the developmental mechanism of steroid-associated osteonecrosis in the femur. In the present study Japanese white rabbits (mean weight 3.5kg) were injected into the gluteus with methylprednisolone (MP) 20mg/kg, and killed after 3 days (MP3 group), 5 days (MP5 group), and 14 days (MP14 group) (n=3 each). As a Control group (C group) Japanese white rabbits not administered MP were used. In experiment 1, the expression of the antioxidant enzymes Superoxide dismutade (SOD) and catalase was compared in liver, kidney, heart, humerus, and femur in C group, and the presence/absence of mitochondria transcription factor A (TFAM) expression was determined by Western blotting (WB) and used to evaluate the number of mitochondria and their function. In experiment 2, the presence/absence of necrosis was determined by immunohistochemistry, while changes in the expression of SOD, catalase, and TFAM in the femur after steroid administration were determined by Western blotting (WB). In experiment 1, intense expression of all of SOD, catalase, and TFAM was found in the liver, kidney, and heart as compared to the humerus and femur. In experiment 2, the expression of all of SOD, catalase, and TFAM in MP3 and MP5 groups was decreased on WB as compared with C group, while in MP14 group a tendency to improvement was seen. Accordingly, steroid-associated mitochondrial injury and redox failure are concluded to be important elements implicated in the pathogenesis of osteonecrosis.

Keywords: transcription factor A (TFAM), Superoxide dismutase (SOD), catalase, steroid-associated osteonecrosis, redox

Citation styles

APA

Tsuchiya, M., Ichiseki, T., Ueda, S., Ueda, Y., Shimazaki, M., Kaneuji, A., Kawahara, N. (2018). Mitochondrial stress and redox failure in steroid-associated osteonecrosis. International Journal of Medical Sciences, 15(3), 205-209. https://doi.org/10.7150/ijms.22525.

ACS

Tsuchiya, M.; Ichiseki, T.; Ueda, S.; Ueda, Y.; Shimazaki, M.; Kaneuji, A.; Kawahara, N. Mitochondrial stress and redox failure in steroid-associated osteonecrosis. Int. J. Med. Sci. 2018, 15 (3), 205-209. DOI: 10.7150/ijms.22525.

NLM

CSE

Tsuchiya M, Ichiseki T, Ueda S, Ueda Y, Shimazaki M, Kaneuji A, Kawahara N. 2018. Mitochondrial stress and redox failure in steroid-associated osteonecrosis. Int J Med Sci. 15(3):205-209.

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions.