Int J Med Sci
2017; 14(11):1101-1109.
doi:10.7150/ijms.20104 This issueCite
Research Paper
Selective Killing of Melanoma Cells With Non-Thermal Atmospheric Pressure Plasma and p-FAK Antibody Conjugated Gold Nanoparticles
Byul Bo Ra Choi1, Jeong Hae Choi1, Jin Woo Hong2, Ki Won Song3, Hae June Lee4, Uk Kyu Kim5✉, Gyoo Cheon Kim1✉
1. Department of Oral Anatomy, School of Dentistry, Pusan National University, Yangsan 626-870, Republic of Korea; 2. Department of Korean Internal Medicine, School of Korean Medicine, Pusan National University, Yangsan 626-870, Republic of Korea; 3. Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, Republic of Korea; 4. Department of Electrical Engineering, Pusan National University, Busan 609-735, Republic of Korea; 5. Department of Oral & Maxillofacial Surgery, School of Dentistry, Pusan National University, Yangsan 626-870, Republic of Korea.
✉ Corresponding authors: Uk Kyu Kim, Department of Oral & Maxillofacial Surgery, School of Dentistry, Pusan National University, Yangsan 626-870, Republic of Korea. Email: kuksjsac.kr; Tel: 82-55-360-5112; fax: 82-55-360-5104. Gyoo Cheon Kim, Department of Oral Anatomy, School of Dentistry, Pusan National University, Yangsan 626-870, Republic of Korea. Email: ki91000mac.kr; Tel: 82-51-510-8243; fax: 82-51-510-8241.More
Citation:
Choi BBR, Choi JH, Hong JW, Song KW, Lee HJ, Kim UK, Kim GC. Selective Killing of Melanoma Cells With Non-Thermal Atmospheric Pressure Plasma and p-FAK Antibody Conjugated Gold Nanoparticles. Int J Med Sci 2017; 14(11):1101-1109. doi:10.7150/ijms.20104. https://www.medsci.org/v14p1101.htm
Melanomas are fast growing high-mortality tumors, and specific treatments for melanomas are needed. Melanoma cells overexpress focal adhesion kinase (FAK) compared to normal keratinocytes, and we sought to exploit this difference to create a selectively lethal therapy.
We combined gold nanoparticles (GNP) with antibodies targeting phosphorylated FAK (p-FAK). These conjugates (p-FAK-GNP) entered G361 melanoma cells and bound p-FAK. Treatment with p-FAK-GNP decreased the viability of G361 cells in a time dependent manner by inducing apoptosis. To maximize the preferential killing of G361 cells, non-thermal atmospheric pressure plasma was used to stimulate the GNP within p-FAK-GNP. Combined treatment with plasma and p-FAK-GNP showed much higher lethality against G361 cells than HaCaT keratinocyte cells. The p-FAK-GNP induced apoptosis over 48 hours in G361 cells, whereas plasma and p-FAK-GNP killed G361 cells immediately.
This study demonstrates that combining plasma with p-FAK-GNP results in selective lethality against human melanoma cells.
Choi, B.B.R., Choi, J.H., Hong, J.W., Song, K.W., Lee, H.J., Kim, U.K., Kim, G.C. (2017). Selective Killing of Melanoma Cells With Non-Thermal Atmospheric Pressure Plasma and p-FAK Antibody Conjugated Gold Nanoparticles. International Journal of Medical Sciences, 14(11), 1101-1109. https://doi.org/10.7150/ijms.20104.
ACS
Choi, B.B.R.; Choi, J.H.; Hong, J.W.; Song, K.W.; Lee, H.J.; Kim, U.K.; Kim, G.C. Selective Killing of Melanoma Cells With Non-Thermal Atmospheric Pressure Plasma and p-FAK Antibody Conjugated Gold Nanoparticles. Int. J. Med. Sci. 2017, 14 (11), 1101-1109. DOI: 10.7150/ijms.20104.
NLM
Choi BBR, Choi JH, Hong JW, Song KW, Lee HJ, Kim UK, Kim GC. Selective Killing of Melanoma Cells With Non-Thermal Atmospheric Pressure Plasma and p-FAK Antibody Conjugated Gold Nanoparticles. Int J Med Sci 2017; 14(11):1101-1109. doi:10.7150/ijms.20104. https://www.medsci.org/v14p1101.htm
CSE
Choi BBR, Choi JH, Hong JW, Song KW, Lee HJ, Kim UK, Kim GC. 2017. Selective Killing of Melanoma Cells With Non-Thermal Atmospheric Pressure Plasma and p-FAK Antibody Conjugated Gold Nanoparticles. Int J Med Sci. 14(11):1101-1109.
This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions.