Effect of Low-Level Laser Therapy on Incorporation of Block Allografts

Valiati, Renato; Paes, Jefferson Viapiana; de Moraes, Aury Nunes; Gava, Aldo; Agostini, Michelle; Masiero, Anelise Viapiana; de Oliveira, Marilia Gerhardt; Pagnoncelli, Rogério Miranda

doi:10.7150/ijms.4440

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Int J Med Sci 2012; 9(10):853-861. doi:10.7150/ijms.4440 This issue Cite

Research Paper

Effect of Low-Level Laser Therapy on Incorporation of Block Allografts

Renato Valiati^1,2✉, Jefferson Viapiana Paes^1,2, Aury Nunes de Moraes³, Aldo Gava³, Michelle Agostini¹, Anelise Viapiana Masiero¹, Marilia Gerhardt de Oliveira⁴, Rogério Miranda Pagnoncelli⁵

1. School of Dentistry, Universidade do Planalto Catarinense (UNIPLAC), Lages, Brazil;
2. School of Dentistry, Pontifícia Universidade Católica do Rio Grande do Sul (PUC-RS), Porto Alegre, Brazil;
3. Universidade do Estado de Santa Catarina (UDESC), Lages, Brazil;
4. Oral and maxillofacial surgery department, PUC-RS, Porto Alegre, Brazil;
5. Dental surgery department, PUC-RS, Porto Alegre, Brazil.

Citation:

Valiati R, Paes JV, de Moraes AN, Gava A, Agostini M, Masiero AV, de Oliveira MG, Pagnoncelli RM. Effect of Low-Level Laser Therapy on Incorporation of Block Allografts. Int J Med Sci 2012; 9(10):853-861. doi:10.7150/ijms.4440. https://www.medsci.org/v09p0853.htm

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Abstract

Objective To assess the effect of low-level laser therapy (LLLT) on the incorporation of deep-frozen block allografts in a rabbit model.

Background Data Studies have shown that LLLT has beneficial effects on tissue repair and new bone formation.

Methods Bone tissue was harvested from two rabbits, processed by deep-freezing and grafted into the calvaria of 12 animals, which were then randomly allocated into two groups: experimental (L) and control (C). Rabbits in group L were irradiated with an aluminum gallium arsenide diode laser (AlGaAs; wavelength 830 nm, 4 J/cm²), applied to four sites on the calvaria, for a total dose of 16 J/cm² per session. The total treatment dose after eight sessions was 128 J/cm². Animals were euthanized at 35 (n = 6) or 70 days (n = 6) postoperatively.

Results Deep-freeze-processed block allografts followed by LLLT showed incorporation at the graft-host interface, moderate bone remodeling, partial filling of osteocyte lacunae, less inflammatory infiltrate in the early postoperative period, and higher collagen deposition than the control group.

Conclusion Optical microscopy and scanning electron microscopy showed that allograft bone processed by deep-freezing plus LLLT is suitable as an alternative for the treatment of bone defects. Use of the deep-freezing method for processing of bone grafts preserves the structural and osteoconductive characteristics of bone tissue.

Keywords: Bone healing, Diode/diode laser, Effect of lasers in tissue, Low-level laser therapy.

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APA

Valiati, R., Paes, J.V., de Moraes, A.N., Gava, A., Agostini, M., Masiero, A.V., de Oliveira, M.G., Pagnoncelli, R.M. (2012). Effect of Low-Level Laser Therapy on Incorporation of Block Allografts. International Journal of Medical Sciences, 9(10), 853-861. https://doi.org/10.7150/ijms.4440.

ACS

Valiati, R.; Paes, J.V.; de Moraes, A.N.; Gava, A.; Agostini, M.; Masiero, A.V.; de Oliveira, M.G.; Pagnoncelli, R.M. Effect of Low-Level Laser Therapy on Incorporation of Block Allografts. Int. J. Med. Sci. 2012, 9 (10), 853-861. DOI: 10.7150/ijms.4440.

NLM

CSE

Valiati R, Paes JV, de Moraes AN, Gava A, Agostini M, Masiero AV, de Oliveira MG, Pagnoncelli RM. 2012. Effect of Low-Level Laser Therapy on Incorporation of Block Allografts. Int J Med Sci. 9(10):853-861.

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