Int J Med Sci 2012; 9(2):157-162. doi:10.7150/ijms.3880 This issue Cite

Research Paper

Inhaled Nitric Oxide Therapy Fails to Improve Outcome in Experimental Severe Influenza

Ilyse Darwish1,2, Chris Miller3, Kevin C. Kain1,2,4, W. Conrad Liles1,2,4 ✉

1. Institute of Medical Science, University of Toronto, Toronto, Canada;
2. Sandra A. Rotman Laboratories, McLaughlin-Rotman Centre for Global Health, University Health Network-Toronto General Hospital, University of Toronto, Toronto;
3. Division of Respiratory Medicine, Department of Medicine, University of British Columbia, Vancouver, Canada;
4. Division of Infectious Diseases, Department of Medicine, University of Toronto, Toronto, Canada.

Citation:
Darwish I, Miller C, Kain KC, Liles WC. Inhaled Nitric Oxide Therapy Fails to Improve Outcome in Experimental Severe Influenza. Int J Med Sci 2012; 9(2):157-162. doi:10.7150/ijms.3880. https://www.medsci.org/v09p0157.htm
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Abstract

In vitro, nitric oxide (NO) has been shown to have antimicrobial activity against a wide range of viruses, including influenza A virus. Therefore, we hypothesized that inhaled nitric oxide (iNO) would increase survival in vivo by reducing the viral load in C57Bl/6 mice infected with a lethal dose of influenza A/WSN/33 (H1N1; WSN/33) virus. NO was delivered to influenza-infected mice either continuously or intermittently at 80 or 160 ppm, respectively, using both prophylactic and post-infection treatment strategies. Murine survival and weight loss were assessed, and lung viral load was quantified via plaque assay. Here, we report that iNO administered prophylactically or post-influenza infection failed to improve survival of infected mice. No difference in lung viral load was observed between experimental groups. Although NO has antiviral activity against influenza A virus in vitro, iNO therapy provided no apparent benefit when used for treatment of influenza A virus infection in vivo.

Keywords: nitric oxide, severe influenza, influenza A/WSN/33


Citation styles

APA
Darwish, I., Miller, C., Kain, K.C., Liles, W.C. (2012). Inhaled Nitric Oxide Therapy Fails to Improve Outcome in Experimental Severe Influenza. International Journal of Medical Sciences, 9(2), 157-162. https://doi.org/10.7150/ijms.3880.

ACS
Darwish, I.; Miller, C.; Kain, K.C.; Liles, W.C. Inhaled Nitric Oxide Therapy Fails to Improve Outcome in Experimental Severe Influenza. Int. J. Med. Sci. 2012, 9 (2), 157-162. DOI: 10.7150/ijms.3880.

NLM
Darwish I, Miller C, Kain KC, Liles WC. Inhaled Nitric Oxide Therapy Fails to Improve Outcome in Experimental Severe Influenza. Int J Med Sci 2012; 9(2):157-162. doi:10.7150/ijms.3880. https://www.medsci.org/v09p0157.htm

CSE
Darwish I, Miller C, Kain KC, Liles WC. 2012. Inhaled Nitric Oxide Therapy Fails to Improve Outcome in Experimental Severe Influenza. Int J Med Sci. 9(2):157-162.

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