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Int J Med Sci 2005; 2(2):64-69. doi:10.7150/ijms.2.64 This issue Cite

Research Paper

Cell Cycle Arrest by a Natural Product via G2/M Checkpoint

Sharon Chui-Wah Luk, Stephanie Wing-Fai Siu, Chun-Kit Lai, Ying-Jye Wu, Shiu-Fun Pang

Technology Development, CK Life Sciences Int'l Inc., 2 Dai Fu Street, Tai Po Industrial Estate, Hong Kong, China

Citation:
Luk SCW, Siu SWF, Lai CK, Wu YJ, Pang SF. Cell Cycle Arrest by a Natural Product via G2/M Checkpoint. Int J Med Sci 2005; 2(2):64-69. doi:10.7150/ijms.2.64. https://www.medsci.org/v02p0064.htm
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Abstract

CKBM is a natural product that exhibits a novel anti-tumor activity through the induction of cell cycle arrest and apoptosis. We have investigated its effects on cell cycle regulation using a gastric cancer cell line, AGS. The effects of CKBM on cell proliferation, cell cycle regulation and apoptosis were analyzed using BrdU (5-bromo-2'-deoxyuridine) cell proliferation assay and flow cytometric analysis, respectively. Specific cellular protein expressions were measured using Western blot analysis. Flow cytometric analysis indicated that CKBM induced G2/M cell cycle arrest and apoptosis, whereas differential protein expressions of p21, p53 and 14-3-3σ (stratifin) using Western blot analysis were enhanced. The differential expressions of p21, p53 and 14-3-3σ in AGS cancer cells after CKBM treatment may play critical roles in the G2/M cell cycle arrest that blocks cell proliferation and induces apoptosis.

Keywords: 14-3-3σ (stratifin), G2/M arrest, cell proliferation, checkpoint protein

Citation styles

APA
Luk, S.C.W., Siu, S.W.F., Lai, C.K., Wu, Y.J., Pang, S.F. (2005). Cell Cycle Arrest by a Natural Product via G2/M Checkpoint. International Journal of Medical Sciences, 2(2), 64-69. https://doi.org/10.7150/ijms.2.64.

ACS
Luk, S.C.W.; Siu, S.W.F.; Lai, C.K.; Wu, Y.J.; Pang, S.F. Cell Cycle Arrest by a Natural Product via G2/M Checkpoint. Int. J. Med. Sci. 2005, 2 (2), 64-69. DOI: 10.7150/ijms.2.64.

NLM
Luk SCW, Siu SWF, Lai CK, Wu YJ, Pang SF. Cell Cycle Arrest by a Natural Product via G2/M Checkpoint. Int J Med Sci 2005; 2(2):64-69. doi:10.7150/ijms.2.64. https://www.medsci.org/v02p0064.htm

CSE
Luk SCW, Siu SWF, Lai CK, Wu YJ, Pang SF. 2005. Cell Cycle Arrest by a Natural Product via G2/M Checkpoint. Int J Med Sci. 2(2):64-69.

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