Low Expression of GIGYF1 Inhibits Metastasis, Proliferation, and Promotes Apoptosis and Autophagy of Gastric Cancer Cells

Zhu, Linqi; Yao, Zhendong; Luo, Qingxin; Liu, Yun; Zhao, Wenjun; Shao, Chen; Shao, Shihe; Cui, Feilun

doi:10.7150/ijms.82719

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Int J Med Sci 2023; 20(8):1038-1045. doi:10.7150/ijms.82719 This issue Cite

Research Paper

Low Expression of GIGYF1 Inhibits Metastasis, Proliferation, and Promotes Apoptosis and Autophagy of Gastric Cancer Cells

Linqi Zhu^1#, Zhendong Yao^2#, Qingxin Luo³, Yun Liu¹, Wenjun Zhao¹, Chen Shao³, Shihe Shao^1,2✉, Feilun Cui^1✉

1. The Affiliated People's Hospital of Jiangsu University. 212002 Zhenjiang, Jiangsu, China.
2. The Affiliated Yixing Hospital of Jiangsu University. 214200 Wu Xi, Jiangsu, China.
3. The Affiliated Hospital of Jiangsu University. 212008 Zhenjiang, Jiangsu, China.
#Linqi Zhu and Zhendong Yao have contributed equally to this work.

Citation:

Zhu L, Yao Z, Luo Q, Liu Y, Zhao W, Shao C, Shao S, Cui F. Low Expression of GIGYF1 Inhibits Metastasis, Proliferation, and Promotes Apoptosis and Autophagy of Gastric Cancer Cells. Int J Med Sci 2023; 20(8):1038-1045. doi:10.7150/ijms.82719. https://www.medsci.org/v20p1038.htm

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Abstract

GRB10 interacting GYF protein 1 (GIGYF1) binds to the N-terminal region of Grb10, regulates multiple signaling pathways. However, it is not clear what happens to cell proliferation, metastasis, apoptosis, and autophagy when the expression level of GIGYF1 gene is reduced. Detection of GIGYF1 expression in clinical tissue specimens and gastric cancer (GC) cell lines by quantitative Real-time PCR (qRT-PCR), GIGYF1 gene was knocked down in MGC-803 cells using small interfering RNA, the effect of GIGYF1 gene on cell metastasis was detected using Transwell assay and wound healing assay, the effect on cell proliferation was detected using plate cloning assay and cck-8 assay, the effect on apoptosis was detected using flow cytometry, autophagosomes were detected using laser confocal microscopy, and the effect on protein expression was detected using immunofluorescence and Western blotting. GIGYF1 gene expression was higher in tumor tissue samples than in paracancer tissue samples, and higher in human GC cell lines than in human normal gastric epithelial cells. GIGYF1 gene knockdown inhibited cell migration, scratch healing ability and EMT process, weakened cell proliferation ability, increased apoptosis rate, promoted the formation of autophagosomes, and changed the corresponding protein expression level. Meanwhile, GIGYF1 knockdowns inhibited the ERK and AKT signaling. In conclusion, the low expression of GIGYF1 gene can inhibit the occurrence and progression of gastric cancer, during which the ERK and AKT signaling pathways are inhibited.

Keywords: GIGYF1, gastric cancer, ERK, AKT

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APA

Zhu, L., Yao, Z., Luo, Q., Liu, Y., Zhao, W., Shao, C., Shao, S., Cui, F. (2023). Low Expression of GIGYF1 Inhibits Metastasis, Proliferation, and Promotes Apoptosis and Autophagy of Gastric Cancer Cells. International Journal of Medical Sciences, 20(8), 1038-1045. https://doi.org/10.7150/ijms.82719.

ACS

Zhu, L.; Yao, Z.; Luo, Q.; Liu, Y.; Zhao, W.; Shao, C.; Shao, S.; Cui, F. Low Expression of GIGYF1 Inhibits Metastasis, Proliferation, and Promotes Apoptosis and Autophagy of Gastric Cancer Cells. Int. J. Med. Sci. 2023, 20 (8), 1038-1045. DOI: 10.7150/ijms.82719.

NLM

CSE

Zhu L, Yao Z, Luo Q, Liu Y, Zhao W, Shao C, Shao S, Cui F. 2023. Low Expression of GIGYF1 Inhibits Metastasis, Proliferation, and Promotes Apoptosis and Autophagy of Gastric Cancer Cells. Int J Med Sci. 20(8):1038-1045.

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