Int J Med Sci 2021; 18(9):2023-2029. doi:10.7150/ijms.51735 This issue

Research Paper

Proteomic analysis of Exosomes derived from the Aqueous Humor of Myopia Patients

Ching-Yao Tsai1,2,3*, Chueh-Tan Chen1,4*, Chin-Hui Lin5, Chen-Chung Liao6, Kate Hua5, Chung-Hua Hsu4,7, Chian-Feng Chen5✉

1. Department of Ophthalmology, Taipei City Hospital, Taipei, Taiwan.
2. MS Program in Transdisciplinary Long Term Care, Fu Jen Catholic University, New Taipei City, Taiwan.
3. Community Medicine Research Center and Institution of Public Health, National Yang Ming Chiao Tung University, Taipei, Taiwan.
4. Institute of Traditional Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan.
5. Cancer Progression Research Center, National Yang Ming Chiao Tung University, Taipei, Taiwan.
6. Metabolomics-Proteomics Research Center, National Yang Ming Chiao Tung University, Taipei, Taiwan.
7. Department of Chinese Medicine, Taipei City Hospital, Linsen, Chinese Medicine, and Kunming Branch, Taipei, Taiwan.
*These authors contributed equally to this study.

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Citation:
Tsai CY, Chen CT, Lin CH, Liao CC, Hua K, Hsu CH, Chen CF. Proteomic analysis of Exosomes derived from the Aqueous Humor of Myopia Patients. Int J Med Sci 2021; 18(9):2023-2029. doi:10.7150/ijms.51735. Available from https://www.medsci.org/v18p2023.htm

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Abstract

Graphic abstract

Objectives: Myopia is the most common refractive vision disorder. In recent years, several studies have suggested that the alteration of the exosomal protein levels in the aqueous humor (AH) is associated with the development of several eye diseases. Therefore, we aimed to explore the exosomal protein profile of the AH from myopia patients.

Methods: Exosomes were isolated from the AH. The quality, concentration, and size distribution of exosomes for each patient were measured using nanoparticle tracking analysis system. Then, the exosomal proteins were purified and digested by trypsin for liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.

Results: There was no significant difference observed between the myopia and control when comparing the concentration and size distribution of exosomes in the AH for each sample. Based on LC-MS/MS analysis, myopia patients had higher and more complex exosomal peptide content. We found two proteins that were common in AH exosomes and eight proteins that were highly expressed in the myopia group.

Conclusions: Our results provide pioneering findings for the exploration of the exosomal protein profile in myopia development. Further studies may provide significant information for the diagnosis, clinical treatment, and prognosis of myopia.

Keywords: aqueous humor, exosome, myopia