Int J Med Sci 2020; 17(17):2635-2643. doi:10.7150/ijms.50097

Research Paper

Pin1 coordinates HDAC6 upregulation with cell migration in lung cancer cells

Hsiang-Hao Chuang1#, Jui-Feng Hsu2,3#, Hsu-Liang Chang2, Pei-Hui Wang1, Po-Ju Wei2, Da-Wei Wu4, Ming-Shyan Huang5,6, Michael Hsiao7✉, Chih-Jen Yang1,8,9✉

1. Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan.
2. Department of Internal Medicine, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan.
3. Division of Hematology and Oncology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan.
4. Department of Internal Medicine, Kaohsiung Municipal Siaogang Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan.
5. Department of Internal Medicine, E-DA Cancer Hospital, Kaohsiung, Taiwan.
6. School of Medicine, I-Shou University, Kaohsiung, Taiwan.
7. Genomics Research Center, Academia Sinica, Taipei, Taiwan.
8. Department of Respiratory Therapy, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
9. School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
#These authors contributed equally to this work.

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Citation:
Chuang HH, Hsu JF, Chang HL, Wang PH, Wei PJ, Wu DW, Huang MS, Hsiao M, Yang CJ. Pin1 coordinates HDAC6 upregulation with cell migration in lung cancer cells. Int J Med Sci 2020; 17(17):2635-2643. doi:10.7150/ijms.50097. Available from http://www.medsci.org/v17p2635.htm

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Abstract

Histone deacetylase 6 (HDAC6) controls many cellular processes via its catalyzing deacetylation of downstream substrates or interacting with its partner proteins. Dysregulation of HDAC6 signaling links to many diseases. Our previous study has been reported peptidyl-prolyl cis/trans isomerase, and NIMA-interacting 1 (Pin1) involving in HDAC6-mediated cell motility. To gain insight into precisely coordination of HDAC6 and Pin1 in cell migration, shRNA-mediated gene silencing and ectopic expression were applied to manipulate protein expression level to evaluate relationship between HDAC6 and Pin1 expression. Quantitative RT-PCR and the cycloheximide (CHX) chase assay resulted in HDAC6 expression is correlated with Pin1 level in H1299 cells. It hints that the Pin1 increases HDAC6 expression through increased transcripts and posttranslational stabilization. Furthermore, wound healing assay and transwell invasion assay evidenced the contribution of Pin1 on cell motility in H1299 cells. Our data suggest that Pin1 acts as an important regulator to manage HDAC6 expression for cell motility in lung cancer cells.

Keywords: HDAC6, Pin1, protein stability, migration