Int J Med Sci 2019; 16(12):1604-1613. doi:10.7150/ijms.37854

Research Paper

Chaenomeles sinensis Koehne extract suppresses the development of atopic dermatitis-like lesions by regulating cytokine and filaggrin expression in NC/Nga mice

Kyung-Jae Cha1, Chang-Seob Song2, Ji-Sook Lee3, Ayesha Kashif1, Min Hwa Hong1, Geunyeong Kim1, In Sik Kim1,4✉

1. Department of Senior Healthcare, BK21 Plus Program, Graduate School, Eulji University, Daejeon 34824
2. Happybio R&D center, Happybio, Cheongju, Chungcheongbuk-do 28101
3. Department of Clinical Laboratory Science, Wonkwang Health Science University, Iksan, 54538
4. Department of Biomedical Laboratory Science, School of Medicine, Eulji University, Daejeon 34824, Republic of Korea

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Citation:
Cha KJ, Song CS, Lee JS, Kashif A, Hong MH, Kim G, Kim IS. Chaenomeles sinensis Koehne extract suppresses the development of atopic dermatitis-like lesions by regulating cytokine and filaggrin expression in NC/Nga mice. Int J Med Sci 2019; 16(12):1604-1613. doi:10.7150/ijms.37854. Available from http://www.medsci.org/v16p1604.htm

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Abstract

Chaenomeles sinensis Koehne (CS) has been used in a traditional oriental medicine for treating throat diseases, anaphylaxis, viral infection, and inflammation. This study investigated the underlying mechanism of anti-allergic effect of CS. Leaves of CS plants were dried, powdered, and then underwent extraction with DMSO. Both ELISA and western blotting were performed to evaluate cytokine concentration and the expression and activation of filaggrin and JNK. Five-week‐old female NC/Nga mice were used as an AD-like mouse model by treating them with 2,4-dinitrochlorobenzene (DNCB). The secretion of TARC, MCP-1, and IL‐8 is increased by TNF-α and IFN-γ in HaCaT cells, and CS extract inhibited the increased production of TARC, MCP-1, and IL‐8. TNF-α and IFN-γ suppressed filaggrin expression by activating JNK. CS extract recovered the expression of filaggrin decreased by TNF-α and IFN-γ by blocking the activation of JNK. In vivo experiment, CS administration reduced thickening of the epidermis and infiltration of inflammatory cells into the dermis as compared to DNCB treatment. Moreover, the decrease of filaggrin expression due to DNCB treatment was recovered by CS administration. The serum IgE level was decreased by CS treatment. The levels of IL-4, IL-5, IL-13 and eotaxin in mouse splenocytes increased after treatment with concanavalin A, and the secretions of IL-4, IL-5, IL-13 and eotaxin were lower in the CS-treated group than in the DNCB group. These results may contribute to the development of a CS-based drug for the treatment of atopic dermatitis.

Keywords: Chaenomeles sinensis Koehne, Anti-inflammatory effect, Atopic dermatitis, Filaggrin, JNK