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Int J Med Sci 2013; 10(1):8-18. doi:10.7150/ijms.5385 This issue Cite

Research Paper

The Therapeutic Effect of Human Adult Stem Cells Derived from Adipose Tissue in Endotoxemic Rat Model

Soyoung Shin¹, Yonggoo Kim¹, Sikyoung Jeong^2✉, Sungyoup Hong², Insoo Kim², Woonjeong Lee², Seungphil Choi²

1. Department of Laboratory Medicine, The Catholic University of Korea, College of Medicine, Seoul, Republic of Korea
2. Department of Emergency Medicine, The Catholic University of Korea, College of Medicine, Seoul, Republic of Korea

✉ Corresponding author: Sikyoung Jeong , M.D. Ph D., Department of Emergency Medicine , College of Medicine, The catholic University of Korea, Daejeon St. Mary's Hospital, Daejeon 301-723, Republic of Korea. Telephone: +82-42-220-9115; Fax: +82-42-256-6810; E-mail: sikyoungac.kr More

Abstract

Excessive systemic inflammation following sepsis, trauma or burn could lead to multi-organ damage and death. Bone marrow stromal cells (BMSCs), commonly referred to as mesenchymal stem cells (MSCs), has been studied in several immune-associated diseases in human and animal by modulating the inflammatory response. Adipose tissue derived mesenchymal stem cells (ATSCs), which can be obtained more easily, compared with BMSCs, has emerged as an attractive alternative MSCs source for cell therapy. We investigated the therapeutic effects of human ATSCs (hATSCs) in endotoxemic rat model and their capacity to modulate the inflammatory response. Endotoxemia was induced with Lipopolysaccaride intravenously injection (LPS, 10mg/kg). Animals were divided into the following three groups: (1) saline + saline (n=5), (2) LPS + saline (n=5) and (3) LPS + hATSCs (2x10⁶) (n=5). The administration of LPS caused a consistent systemic inflammatory responses, increased concentrations of the pro-inflammatory cytokines that have an important role in sepsis. Treatment of endotoxemia with hATSCs decreased the level of inflammatory cytokines both in serum and in the lung, reduced inflammatory changes in the lung, prevented apoptosis in the kidney and improved multi-organ injury. In conclusion, our data demonstrates that hATSCs regulate the immue/inflammatory responses and improve multi-organ injury and they could be attractive candidates for cell therapy to treat endotoxemia.

Keywords: Endotoxemia, Adipose tissue derived mesenchymal stem cells(ATSCs)

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