1. Department of Cardiovascular Surgery, Dokuz Eylül University, Faculty of Medicine, 35340 İnciraltı İzmir, TURKEY.
2. Department of Biomechanics, Dokuz Eylül University, Health Sciences Institute, 35340 İnciraltı İzmir, TURKEY.
3. Department of Medical Biology and Genetics, Dokuz Eylül University, Faculty of Medicine, 35340 İnciraltı İzmir, TURKEY.
4. Department of Pathology, Dokuz Eylül University, Faculty of Medicine, 35340 İnciraltı İzmir, TURKEY.
5. Department of Anesthesiology, Dokuz Eylül University, Faculty of Medicine, 35340 İnciraltı İzmir, TURKEY.
* Current address: Section for Medical Expert and Knowledge-Based Systems, Center for Medical Statistics, Informatics, and Intelligent Systems, Medical University of Vienna, Spitalgasse 23 A-1090 Vienna, AUSTRIA
Background: Acute myocardial infarction (AMI) remains as one of the most common lethal diseases in the world and therefore it is necessary to understand its effect on molecular basis. Genome-wide microarray analysis provides us to predict potential biomarkers and signaling pathways for this purpose.
Objectives: The aim of this study is to understand the molecular basis of the immediate right ventricular cellular response to left ventricular AMI.
Material and Methods: A rat model of left anterior descending coronary artery ligation was used to assess the effect of left ventricular AMI on both the right ventricle as a remote zone and the left ventricle as an ischemic/infarct zone. Microarray technology was applied to detect the gene expression. Gene Ontology and KEGG pathways analysis were done to identify effected pathways and related genes.
Results: We found that immune response, cell chemotaxis, inflammation, cytoskeleton organization are significantly deregulated in ischemic zone as early response within 30 min. Unexpectedly, there were several affected signaling pathways such as cell chemotaxis, regulation of endothelial cell proliferation, and regulation of caveolea regulation of anti-apoptosis, regulation of cytoskeleton organization and cell adhesion on the remote zone in the right ventricle.
Conclusion: This data demonstrates that there is an immediate molecular response in both ventricles after an AMI. Although the ischemia did not histologically involve the right ventricle; there is a clear molecular response to the infarct in the left ventricle. This provides us new insights to understand molecular mechanisms behind AMI and to find more effective drug targets.
Keywords: Acute myocardial infarction, molecular response