Characteristics of in peripheral blood of 70 hospitalized patients and 8 diarrhea patients with COVID-19

Objective: To analyze the blood test indicators of patients after infection of COVID-19 in Chongqing and analyze the clinical indicators of 8 patients with diarrhea. Materials and Methods: From January 26, 2019 to February 13, 2020, 70 patients diagnosed with 2019-nCoV according to the World Health Organization interim guidance for NCP and divided into diarrhea and non-diarrhea groups. The laboratory tests liver and kidney function, blood routine, coagulation function, and immune status. Results: The study population included 70 hospitalized patients with confirmed CONV-2019. NCP patients (43males and 27 females) with a mean age of 48.57±17.80 (9~82) years and only 4.3% of patients have lung-related diseases. The positive rate of ESR, CRP, PT, IL6, lymphocyte count, GGT, Prealbumin and CD4 was more than 50%. We further analyzed the differences between 8 diarrhea patients and 62 non-diarrhea patients. Among these indicators, only Lymphocyte, CRP, Prealbumin and Cystatin C positive rate is more than 50%. Although there is no statistical difference in GGT, 100% of the 7 patients tested decreased. Conclusion: Our data recommended that the ESR, CRP, PT, IL6, lymphocyte count, GGT, prealbumin and CD4 have important value in the diagnosis of COVID-19, and the decrease of GGT may be an important indicator for judging the intestinal dysfunction of patients.


Introduction
The new coronavirus (CoV) named by the World Health Organization (WHO) as "COVID-19" led to a pneumonia outbreak that recently started in Wuhan, Hubei Province China [1]. Its outbreak was linked to a large seafood and animal market and investigations are underway to determine the source of infection. Thousands of human infections and many export cases worldwide have been confirmed in China to date [2]. The main source of infection is pneumonia in patients infected with new coronavirus. Respiratory droplets are the main route of transmission and can also be transmitted through contact. People are often vulnerable. The main symptoms are respiratory symptoms such as fever and cough. However, recently, Professor An Ping et al. observed that the first symptom of some patients with pneumonia infected by the new coronavirus is only diarrhea, and it is suspected that the digestive system may also transmit the new coronavirus. At present, the mechanism by which digestive symptoms appear is unclear. The purpose of this study was to analyze the basic conditions of 70 patients with new-type coronavirus in Yongchuan Hospital, to analyze the blood test indicators of patients after infection, and

Ivyspring
International Publisher the differences in the detection indexes of the infected people in Wuhan and Chongqing, and to analyze the clinical indicators of 8 patients with diarrhea. This will assist in the ability to find specific clinical testing indicators for patients with diarrhea.

Patients
From January 26, 2019 to February 13, 2020, 70 patients diagnosed with 2019-nCoV according to the World Health Organization interim guidance for NCP and divided into diarrhea and non-diarrhea groups. All patients were confirmed to be positive for new coronavirus nucleic acid by real-time fluorescent RT-PCR. Infection samples were collected from Chongqing Hospital.

Blood sampling
Blood samples of the patients were collected by the nurse according to the doctor's order, and all patients were not treated before the blood sampling or did not receive the standardized treatment according to the diagnosis and treatment scheme of NCP.

Blood test
Liver and kidney function, immunoglobulin complement, and fructosamine machines are all Hitachi 7600 automatic biochemical analyzers from Japan. The blood routine is a Japanese Sysmex XN-1000 automatic blood analyzer. The coagulation test is a Japanese Sysmex CA7000 automatic coagulation analyzer. Glycated hemoglobin is a Mindray H50 glycated hemoglobin analyzer. Erythrocyte sedimentation is an instrument that I haven't noticed, and I haven't changed it yet.

Statistical analysis
All statistical analyses were performed using SPSS 20.0 (SPSS Inc., Chicago, IL, USA). Descriptive analyses were performed for categorical variables such as gender. Continuous variables such as inspection results were expressed as x ± s and compared using the independent samples t-test. P<0.05 was considered as statistically significant.

Clinical characteristics of all patients
All patents' clinical specimens for CONV-2019 diagnostic testing were obtained in accordance with the guidelines of the CDC [3,4]. A description of the assay and sequence information for the RT-PCR primers and probes are available on the CDC Laboratory Information website for CONV-2019.
The study population included 70 hospitalized patients with confirmed CONV-2019. NCP patients (43males and 27 females) with a mean age of 48.57±17.80 (9~82) years and only 4.3% of patients have lung-related diseases. Lesion site locations were prominent in both lungs (Table 1).

Comparison of diarrhea and non-diarrhea populations
However, the clinical examination of patients with diarrhea as the main symptom is not clear. We further analyzed the differences between 8 diarrhea patients and 62 non-diarrhea patients. The Eight patients had diarrhea as the first symptom, and the throat swab test was negative but the anal swab test was positive, and except for one patient who had slight inflammation of the lungs when admitted, the other patients had no obvious inflammatory changes in the lungs (Figure 1). There was no significant difference between the two groups in the general situation of age, gender, medical history, etc. (Table  3). Comparison of clinical test indicators between the two groups of patients found that GGT (diarrhea vs. non-diarrhea: 20.75±14.12 vs. 54.74±59.61; P=0.000) was significantly different. The results indicate that GGT may be a deliberate detection index for patients with diarrhea. Further analysis of clinical test indicators of 8 patients showed that the Lymphocyte (4/8), PLT(2/8), CRP (4/8), PT (2/8), Prealbumin (5/8), Creatinine (2/8), Cystatin C (4/8), C4 (3/8) and CD8 (3/8) had significant differences (P<0.05). Among these indicators, only Lymphocyte, CRP, Prealbumin and Cystatin C positive rate is more than 50%. Although there is no statistical difference in GGT, 100% of the 7 patients tested decreased, so it further illustrates the important detection value of GGT in patients with diarrhea.

Discussion
So far, there is no vaccine or specific antiviral treatment for this emerging infectious disease, and effective control still depends mainly on early diagnosis, patient isolation and close contact monitoring [3]. Therefore, reliable and accurate diagnostic methods play a vital role in the field of disease control and prevention. However, some patients with diarrhea have a false-negative nucleic acid test. So, finding indicators that can predict gut symptoms in patients has important clinical significance. Our data found that the positive rate of ESR, CRP, PT, IL6, lymphocyte count, GGT, Prealbumin and CD4 was more than 50%. In addition, it was found that the Lymphocyte, PLT, CRP, PT, Prealbumin, Creatinine, Cystatin C, C4 and CD8 have clinical significance for patients in the diagnosis of diarrhea. GGT reduction is probably the most important indicator. Taken together, consistent with previous research [2,5], the ESR, CRP, PT, IL6, lymphocyte count, GGT, Prealbumin and CD4 have important value in the diagnosis of COVID-19, and the decrease of GGT may be an important indicator for judging the intestinal dysfunction of patients.

The data availability statement
All data gathered and included in the present study are available and can be provided by the corresponding author upon reasonable request.

Ethics approval
This study was approved by the ethical committee of Yongchuan Hospital of Chongqing Medical University and informed consent was obtained from each patient.