Int J Med Sci 2019; 16(9):1287-1294. doi:10.7150/ijms.36265

Research Paper

Impact of general anaesthesia on endoplasmic reticulum stress: propofol vs. isoflurane

Eun-Hye Seo1*, Liyun Piao2*, Hyun-Jun Park2, Ji Yeon Lee2, Mijung Sa3, Chung-Sik Oh3, Seung-Hyun Lee4,5, Seong-Hyop Kim2,3,5✉

1. BK21 Plus, Department of Cellular and Molecular Medicine, Konkuk University School of Medicine, Seoul, Korea.
2. Department of Infection and Immunology, Konkuk University School of Medicine, Seoul, Korea.
3. Department of Anesthesiology and Pain medicine, Konkuk University Medical Center, Konkuk University School of Medicine, Seoul, Korea.
4. Department of Microbiology, Konkuk University School of Medicine, Seoul, Korea.
5. Department of Medicine, Institute of Biomedical Science and Technology, Konkuk University School of Medicine, Seoul, Korea.
*The two authors evenly contributed to the study as first author.

Abstract

Background: This study investigated the effects of propofol and isoflurane on endoplasmic reticulum (ER) stress in an animal model under general anaesthesia.

Methods: Rats were randomly divided into Propofol and Isoflurane groups. Anaesthesia was maintained with propofol for Propofol group or isoflurane for Isoflurane group during 3 h. ER stress from lymphocytes in blood and tissues was evaluated between two groups after euthanasia. Reactive oxygen species (ROS) from lymphocytes in blood and tissues, and cytokines in blood were also checked. An immunohistochemical assay for ER stress marker from tissues was performed.

Results: After anaesthesia, the levels of CCAAT-enhancer-binding protein homologous proteins (CHOP) in blood and liver were significantly higher in Isoflurane group, compared to Propofol group [blood, 31,499 ± 4,934 (30,733, 26,441-38,807) mean fluorescence intensity (MFI) in Isoflurane group vs. 20,595 ± 1,838 (20,780, 18,866-22,232) MFI in Propofol group, p = 0.002; liver, 28,342 ± 5,535 (29,421, 23,388-32,756) MFI in Isoflurane group vs. 20,004 ± 2,155 (19,244, 18,197-22,191) MFI in Propofol group, p = 0.020]. ROS in blood was significantly higher in Isoflurane group, compared to Propofol group. However, cytokines in blood and immunohistochemical assays in tissues were similar between groups.

Conclusion: Significant higher of ER stress from blood and liver were observed in rats under anaesthesia with isoflurane, compared to those that received propofol. ROS from blood also showed significant higher under anaesthesia with isoflurane. However, these findings were not associated with any changes in cytokines in blood or immunohistochemical assay in tissues.

Keywords: Propofol, Isoflurane, Endoplasmic reticulum stress, Reactive oxygen species

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How to cite this article:
Seo EH, Piao L, Park HJ, Lee JY, Sa M, Oh CS, Lee SH, Kim SH. Impact of general anaesthesia on endoplasmic reticulum stress: propofol vs. isoflurane. Int J Med Sci 2019; 16(9):1287-1294. doi:10.7150/ijms.36265. Available from http://www.medsci.org/v16p1287.htm