Int J Med Sci 2019; 16(9):1254-1259. doi:10.7150/ijms.35984

Research Paper

Protein chip and bioinformatic analyses of differentially expressed proteins involved in the effect of hydrogen-rich water on myocardial ischemia-reperfusion injury

Liangtong Li1,4,*, Tongtong Liu2,*, Xiangzi Li1, Xuanchen Liu1, Li Liu1, Shaochun Li1, Zhilin Li3, Yujuan Zhou1✉, Fulin Liu2✉

1. School of Medicine, Hebei University, Baoding, 071000, China
2. Affiliated Hospital of Hebei University, Baoding, 071000, China
3. School of Chemistry, Hebei University, Baoding 071000, China
4. Central Laboratory of Affiliated Hospital of Hebei University, Baoding 071000, China
5. Department of Cardiac Surgery, Affiliated Hospital of Hebei University, Baoding, 071000, China
*These authors contributed equally to this work.

Abstract

Background: The differentially expressed proteins (DEPs) involved in the effect of hydrogen-rich water on myocardial ischemia reperfusion injury (MIRI) and their biological processes and signaling pathway were analyzed. Methods: 20 Wistar rats were randomly and equally divided into a control and a hydrogen-rich group. Hearts were removed and fixed in a Langendorff device. The control group was perfused with K-R solution, and the hydrogen-rich water group was perfused with K-R solution + hydrogen-rich water. Protein was extracted from the ventricular tissues, and GSR-CAA-67 was used to identify the DEPs between two groups. DEPs were analyzed through bioinformatic methods. Results: Compared with the control group, in the treatment group, the expression of 25 proteins was obviously decreased (P<0.05). For the DEPs, 359 biological processes, including the regulation of signaling pathways, immune reaction and formation of cardiovascular endothelial cells, were selected by GO enrichment analysis. Five signaling pathways were selected by KEGG pathway enrichment analysis. Conclusions: 25 proteins that are involved in hydrogen-water reducing MIRI were selected by high-throughput GSR-CAA-67. The biological processes and metabolic pathways involved in the DEPs were summarized, providing theoretical evidence for the clinical application of hydrogen-rich water.

Keywords: hydrogen-rich water, myocardial ischemia-reperfusion injury, protein chip, GO enrichment analysis, KEGG pathway analysis

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How to cite this article:
Li L, Liu T, Li X, Liu X, Liu L, Li S, Li Z, Zhou Y, Liu F. Protein chip and bioinformatic analyses of differentially expressed proteins involved in the effect of hydrogen-rich water on myocardial ischemia-reperfusion injury. Int J Med Sci 2019; 16(9):1254-1259. doi:10.7150/ijms.35984. Available from http://www.medsci.org/v16p1254.htm