Int J Med Sci 2019; 16(9):1207-1214. doi:10.7150/ijms.36131

Research Paper

Parvalbumin Promoter Methylation Altered in Major Depressive Disorder

Benjamard Thaweethee-Sukjai1,2, Sirijit Suttajit3, Samur Thanoi1,2, Caroline F. Dalton4, Gavin P. Reynolds2,4, Sutisa Nudmamud-Thanoi1,2✉

1. Department of Anatomy, Faculty of Medical Science, Naresuan University, Phitsanulok, 65000, Thailand
2. Centre of Excellence in Medical Biotechnology, Faculty of Medical Science, Naresuan University, Phitsanulok, 65000, Thailand
3. Department of Psychiatry, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand
4. Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield, S1 1WB, UK

Abstract

Aims: To determine the extent of DNA methylation of parvalbumin gene (PVALB) promoter in major depressive disorder (MDD) patients with and without suicide attempt in comparison with healthy controls.

Methods: The extracted DNA from dried blood spots of MDD patients (n = 92) including non-suicidal MDD and suicidal-MDD subgroups (n = 45 and n = 47, respectively) and age-matched control subjects (n = 95) was used for DNA methylation analysis at four CpG sites in the promoter sequence of PVALB by pyrosequencing.

Results: The PVALB methylation was significantly increased at CpG2 and decreased at CpG4 in the MDD group compared to the control group, while there was no difference between non-suicidal MDD and suicidal-MDD subgroups. A significant inverse correlation of severity of MDD was indicated only for CpG4.

Conclusion: This study provides the first evidence of abnormalities of PVALB promoter methylation in MDD and its correlation with MDD severity indicating a role for epigenetics in this psychiatric disorder.

Keywords: DNA methylation, major depressive disorder, parvalbumin, pyrosequencing, suicide attempt

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How to cite this article:
Thaweethee-Sukjai B, Suttajit S, Thanoi S, Dalton CF, Reynolds GP, Nudmamud-Thanoi S. Parvalbumin Promoter Methylation Altered in Major Depressive Disorder. Int J Med Sci 2019; 16(9):1207-1214. doi:10.7150/ijms.36131. Available from http://www.medsci.org/v16p1207.htm