27 May 2019
Global reach, higher impact
Int J Med Sci 2019; 16(2):189-197. doi:10.7150/ijms.30647
Antarctic freshwater microalga, Chloromonas reticulata, suppresses inflammation and carcinogenesis
1. Department of Bioscience, Mokpo National University, Muan 58554, Republic of Korea
Inflammation triggered by the innate immune system is a strategy to protect organisms from the risk of environmental infection. However, it has recently become clear that inflammation can cause a variety of human diseases, including cancer. In this study, we investigated the effects of an ethanol extract of the Antarctic freshwater microalgae, Chloromonas reticulata (ETCH), on inflammation and carcinogenesis in RAW 264.7 macrophages and HCT116 human colon cancer cells, respectively. ETCH exhibited significant anti-inflammatory activity through the dose-dependent modulation of major inflammatory markers such as COX-2, IL-6, iNOS, TNF-α, and NO production. For example, ETCH reduced LPS-induced upregulation of COX-2, IL-6, iNOS, and TNF- alpha mRNA levels, leading to a significant decrease in the levels of LPS-stimulated NO and IL-6 as well as TNF-alpha products. In contract, ETCH exhibited dose-dependent cytotoxic activity against HCT116 cells, yielding a profound reduction in the proliferation of the cancer cells. Furthermore, ETCH induced G2 phase cell cycle arrest by transcriptionally regulating of genes involved in G2 / M transition including p21 (CDKN1A), cyclin B1 (CCNB1), and CDK1; CDKN1A mRNA levels were upregulated in response to ETCH, whereas CCNB1 and CDK1 were downregulated. This study reports for the first time anti-inflammatory and anti-cancer effects of, C. reticulata and provides new insights into the molecular mechanisms of the linkage between inflammation and cancer.
Keywords: Inflammation cancer, Chloromonas reticulata, pro-inflammatory cytokines, HCT116
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How to cite this article:
Suh SS, Hong JM, Kim EJ, Jung SW, Chae H, Kim JE, Kim JH, Kim IC, Kim S. Antarctic freshwater microalga, Chloromonas reticulata, suppresses inflammation and carcinogenesis. Int J Med Sci 2019; 16(2):189-197. doi:10.7150/ijms.30647. Available from http://www.medsci.org/v16p0189.htm