International Journal of Medical Sciences

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18 October 2018

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Int J Med Sci 2018; 15(13):1473-1479. doi:10.7150/ijms.26657

Research Paper

Borrelia burgdorferi basic membrane protein A stimulates murine macrophage to secrete specific chemokines

Yun Peng 1*, Zhang Liang3,4*, Aihua Liu2,3,4,5,6 ✉, Erhua Li1, Xiting Dai1, Ruolan Bai2, Zhenhua Ji1, Miaomiao Jian2, Mingbiao Ma1, Lvyan Tao2, Fukai Bao1,2,4,5,6 ✉, Feng Wang1, YunFeng Bi1, Zhe Ding1, Abi Manzama-Esso1

1. Department of Microbiology and Immunology, Kunming Medical University, Kunming 650500, China,
2. Department of Biochemistry and Molecular Biology, Kunming Medical University, Kunming 650500, China,
3. Yunnan Province Key Laboratory for Tropical Infectious Diseases in Universities, Kunming Medical University, Kunming 650500, China,
4. The Institute for Tropical Medicine, Kunming Medical University, Kunming 650500, China,
5. Yunnan Demonstration Base of International Science and Technology Cooperation for Tropical Diseases, Kunming 650500, China,
6. Yunnan Province Integrative Innovation Center for Public Health, Diseases Prevention and Control, Kunming Medical University, Kunming 650500, China.
*These authors contributed equally to the study.

Abstract

In this study, we investigated the mechanisms that lead to the production of proinflammatory mediators by the murine macrophage cell line, RAW264.7, when these cells are exposed in vitro to recombinant Borrelia burgdorferi basic membrane protein A (rBmpA). Using antibody protein microarray technology with high-throughput detection ability for detecting 25 chemokines in culture supernatant the RAW264.7 cell culture supernatants at 12 and 24 h post-stimulation with rBmpA, we identified two chemokines, a monocyte chemoattractant protein-5 (MCP-5/CCL12) and a macrophage inflammatory protein-2 (MIP-2/CXCL2), both of which increased significantly after stimulation. We then chose these two chemokines for further study. Enzyme-linked immunosorbent assay and real-time polymerase chain reaction revealed that with the increase of rBmpA concentration, MCP-5/CCL12 and MIP-2/CXCL2 showed concentration-dependent increases (p <0.01).Our results indicate that the rBmpA could stimulate the secretion of several specific chemokines and induce Lyme arthritis.

Keywords: Borrelia burgdorferi, basic membrane protein A (rBmpA), murine macrophage cell line, RAW264.7, chemokine, Lyme arthritis

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How to cite this article:
Peng Y, Liang Z, Liu A, Li E, Dai X, Bai R, Ji Z, Jian M, Ma M, Tao L, Bao F, Wang F, Bi Y, Ding Z, Manzama-Esso A. Borrelia burgdorferi basic membrane protein A stimulates murine macrophage to secrete specific chemokines. Int J Med Sci 2018; 15(13):1473-1479. doi:10.7150/ijms.26657. Available from http://www.medsci.org/v15p1473.htm