International Journal of Medical Sciences

Impact factor
2.284

17 August 2018

ISSN 1449-1907 News feeds of published articles

Manuscript login | Account

open access Global reach, higher impact

Theranostics

Journal of Cancer

International Journal of Biological Sciences

Journal of Genomics

Journal of Bone and Joint Infection (JBJI)

Oncomedicine

Journal of Biomedicine

Nanotheranostics

PubMed Central Indexed in Journal Impact Factor

Int J Med Sci 2018; 15(12):1349-1354. doi:10.7150/ijms.27609

Research Paper

GABAergic Alterations in the Rat Testis after Methamphetamine Exposure

Paweena Kaewman1,2, Sutisa Nudmamud-Thanoi1,2, Samur Thanoi1,2✉

1. Department of Anatomy, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand.
2. Centre of Excellence in Medical Biotechnology, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand.

Abstract

Gamma-aminobutyric acid (GABA), GABA-A receptors and GABA transporter 1 (GAT1) were reported to be involved in the proliferation of Leydig cells, testosterone production and spermatogenesis. Since methamphetamine (METH) has been reported to have adverse effects on testis and its functions, the aim of this study was therefore to determine the changes of GABAergic activity in testis after METH exposure. Male Sprague-Dawley rats were divided into control, acute binge (AB-METH), escalating dose (ED METH) and escalating dose-binge (ED-binge METH) groups. After sacrifice, rat testes were removed and used to estimate GABA concentration and the expression of GABA-A receptor, GAD1, GAD2 and GAT1 genes by using HPLC and RT-PCR, respectively. The GABA concentration was significantly increased in all METH-administrated groups. In addition, significant increases of GABA-A α1 receptor and GAD1 genes expression were found in the ED-binge METH group. Gene expressions of GAT1 were numerically decreased in all METH-administrated rats and reached significant in the ED METH group. These results indicated a compensatory upregulation of GABA production and its functions in testis after METH exposure. Thus, these changes might represent a homeostatic response of GABAergic to the adverse effects of METH.

Keywords: Rat testis, Methamphetamine, GABA, GABA-A α1 receptor, Glutamate decarboxylase, GAT1.

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions.
How to cite this article:
Kaewman P, Nudmamud-Thanoi S, Thanoi S. GABAergic Alterations in the Rat Testis after Methamphetamine Exposure. Int J Med Sci 2018; 15(12):1349-1354. doi:10.7150/ijms.27609. Available from http://www.medsci.org/v15p1349.htm