International Journal of Medical Sciences

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23 October 2018

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Int J Med Sci 2018; 15(12):1341-1348. doi:10.7150/ijms.27543

Research Paper

Thrombopoietin could protect cerebral tissue against ischemia-reperfusion injury by suppressing NF-κB and MMP-9 expression in rats

Wenjuan Wu1,2, Wei Zhong1, Bing Lang3, Zhiping Hu1, Jialin He1, Xiangqi Tang1✉

1. Department of Neurology, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China
2. Department of Neurology, The First Affiliated Hospital of Henan University of Science and Technology
3. National Clinical Research Center for Mental Disorders, the Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China
Wenjuan Wu and Wei Zhong contributed equally to this work

Abstract

Objective: To determine the neuroprotective effects and underpinning mechanisms of thrombopoietin (TPO), Matrix Metalloproteinase-9(MMP-9) and Nuclear Factor-κB (NF-κB) after focal cerebral ischemia-reperfusion in rats.

Methods: Male rats underwent 2 hours of right middle cerebral artery occlusion (MCAO) followed by 22 hours of reperfusion. PBS or TPO (0.1ug/kg) was administered from caudal vein before reperfusion. Neurologic deficits, brain edema, Evans blue (EB) extravasation, NF-κB and MMP-9 expression were subsequently examined.

Results: Ischemia-reperfusion injury produced a large area of edema. TPO significantly reduced edema and alleviated neurologic deficits after ischemia-reperfusion. Ischemia-induced increases of NF-κB, MMP-9 and Evans blue extravasation were reduced by TPO intervention.

Conclusion: TPO improved neurological function and ameliorated brain edema after stroke, partly by reducing the ischemia-induced increase of NF-κB and MMP-9.

Keywords: Thrombopoietin (TPO), Nuclear factor-κB (NF-κB), Matrix Metalloproteinase-9 (MMP-9), Ischemia-Reperfusion (IR)

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions.
How to cite this article:
Wu W, Zhong W, Lang B, Hu Z, He J, Tang X. Thrombopoietin could protect cerebral tissue against ischemia-reperfusion injury by suppressing NF-κB and MMP-9 expression in rats. Int J Med Sci 2018; 15(12):1341-1348. doi:10.7150/ijms.27543. Available from http://www.medsci.org/v15p1341.htm