International Journal of Medical Sciences

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24 September 2018

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Int J Med Sci 2018; 15(11):1227-1234. doi:10.7150/ijms.25662

Research Paper

Long non-coding RNA NEAT1 promotes proliferation, migration and invasion of human osteosarcoma cells

Pengcheng Li1, Rui Huang2, Tao Huang1✉, Shuo Cheng1, Yao Chen1, Zhihang Wang1

1. Department of Orthopedics, The First Affiliated Hospital of China Medical University. Shenyang 110001, Liaoning, P.R. China
2. Department of Clinical Medicine, Da Lian Medical University. Dalian 116000, Liaoning, P.R. China.

Abstract

Aim: Long non-coding RNAs (LncRNAs) have been identified to play a crucial role in tumorigenesis and the progression of many types of tumors. However, the clinical significance and biological function of lncRNA nuclear-enriched abundant transcript 1(NEAT1) in human osteosarcoma remains unknown. Here, we investigated the role of NEAT1 in human osteosarcoma cell lines and clinical tumor samples.

Methods: In this study, expression of NEAT1 was analyzed in 19 osteosarcoma tissues and paired adjacent non-tumor tissues by using quantitative real-time PCR. Additionally, knockdown of NEAT1 expression using Lentivirus-mediated siRNA was performed in order to explore the biological function of NEAT1 on osteosarcoma cell proliferation and metastasis through MTT, colony formation assay and transwell assay.

Results: NEAT1 was over-expressed in osteosarcoma tissues compared with adjacent non-tumor tissues. In addition, knockdown of NEAT1 expression could suppress cell proliferation, migration and invasion in vitro.

Conclusion: LncRNA NEAT1 was up-regulated in osteosarcoma tissue, promoting proliferation and metastasis of osteosarcoma cells. These findings indicate the role of this substance, as a growth regulator in osteosarcoma, and thus it may serve as a novel biomarker, and drug target for developing osteosarcoma therapies.

Keywords: NEAT1, Long non-coding RNA, Osteosarcoma

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How to cite this article:
Li P, Huang R, Huang T, Cheng S, Chen Y, Wang Z. Long non-coding RNA NEAT1 promotes proliferation, migration and invasion of human osteosarcoma cells. Int J Med Sci 2018; 15(11):1227-1234. doi:10.7150/ijms.25662. Available from http://www.medsci.org/v15p1227.htm