International Journal of Medical Sciences

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23 October 2018

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Int J Med Sci 2018; 15(7):703-712. doi:10.7150/ijms.21660

Research Paper

Differential Expression of CircRNAs in Embryonic Heart Tissue Associated with Ventricular Septal Defect

Heng Liu1,2#, Yin Hu1,2#, Bin Zhuang1,2, Jing Yin1,2, Xiao-Hui Chen1,2, Juan Wang1,2, Meng-Meng Li1,2, Jing Xu1,2, Xing-Yun Wang1,2, Zhang-Bin Yu1,2✉, Shu-Ping Han1,2✉

1. Department of Pediatrics, The Affiliated Obstetrics and Gynecology Hospital of Nanjing Medical University, No. 123 Tian Fei Xiang, Mo Chou Road, Nanjing 210004, Jiangsu Province, China.
2. Department of Pediatrics, Nanjing Maternity and Child Health Care Hospital, No. 123 Tian Fei Xiang, Mo Chou Road, Nanjing 210004, Jiangsu Province, China.
#These authors contributed equally to this work.

Abstract

Objectives: To explore and validate the differential expression of circRNAs in the myocardium of congenital ventricular septal defect (VSD) and to explore a new avenue of research regarding the pathological mechanisms of VSD.

Methods: We detected circRNAs expression profiles in heart tissues taken from six aborted fetuses with VSD and normal group using circRNA microarray. Some differentially expressed circRNAs were studied by bioinformatics analysis. Finally, quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed to confirm these results.

Results: This study found abundant circRNAs in the myocardium taken from individuals in the normal group and the VSD group. After that, totally 6234 differentially expressed circRNAs between the normal group and the VSD group were confirmed (Fold change ≥ 2.0; p < 0.05). Then, this research carried out bioinformatics analysis and predicted the potential biological functions of circRNAs. Finally, the over-expression of hsa_circRNA_002086 and under-expression of hsa_circRNA_007878, hsa_circRNA_100709, hsa_circRNA_101965, hsa_circRNA_402565 were further validated by qRT-PCR.

Conclusions: There is a significant difference in expression of the circRNA in cardiac tissue from VSD group compared to the normal group. Combined with the microarray results and previous researches, circRNAs may contribute to the occurrence of VSD by acting as miRNA sponges or by binding proteins, these possible roles for circRNAs in VSD require elucidation in additional studies.

Keywords: Congenital Heart Disease (CHD), fetation, heart development, miRNA sponges

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How to cite this article:
Liu H, Hu Y, Zhuang B, Yin J, Chen XH, Wang J, Li MM, Xu J, Wang XY, Yu ZB, Han SP. Differential Expression of CircRNAs in Embryonic Heart Tissue Associated with Ventricular Septal Defect. Int J Med Sci 2018; 15(7):703-712. doi:10.7150/ijms.21660. Available from http://www.medsci.org/v15p0703.htm