15 December 2017
Int J Med Sci 2017; 14(8):758-763. doi:10.7150/ijms.18907
Jak2a regulates erythroid and myeloid hematopoiesis during zebrafish embryogenesis
1. Department of Medical Oncology, Hubei Cancer Hospital, Wuhan, Hubei 430079, China.
Zebrafish (Danio rerio) are an attractive vertebrate model for the molecular dissection of disease mechanisms. Janus kinase (JAK)/signal transducer and activator of transcription (stat) has been defined through studies of cytokine signaling pathways in mammals. Here, we examined the expression level of Jak2a, which is a homolog of mammalian jak2 in zebrafish, by quantitative reverse transcriptase (RT)-PCR, and the peak of mRNA expression occurred at 3.75 hours post fertilization (hpf). The overexpression of Jak2a was proven by real-time Q-PCR and Western blot in 1-4-cell stage embryos injected with 400 ng/µl full-length jak2a mRNA as well as gfi1.1, gata1, mpo and β-embryonic hemoglobin as detected by real-time Q-PCR. Moreover, jak2a mRNA significantly increased the GFP+ population in the transgenic zebrafish lines Tg (gata1: gfp) (uninjected embryos: 17.22±1.70%; embryos injected with jak2a mRNA: 21.31±2.11%, p<0.01) and Tg (mpo: gfp) (uninjected embryos: 3.86±1.94; embryos injected with jak2a mRNA: 6.64±1.30%, p<0.01) compared with the control group. Thus, our data indicate that Jak2a plays an important role in erythropoiesis and myeloid hematopoiesis.
Keywords: zebrafish, Jak2a, mRNA, overexpression, hematopoiesis
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How to cite this article:
Zhu X, Liu R, Guan J, Zeng W, Yin J, Zhang Y. Jak2a regulates erythroid and myeloid hematopoiesis during zebrafish embryogenesis. Int J Med Sci 2017; 14(8):758-763. doi:10.7150/ijms.18907. Available from http://www.medsci.org/v14p0758.htm