International Journal of Medical Sciences
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25 May 2017

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Int J Med Sci 2017; 14(6):530-535. doi:10.7150/ijms.19024

Research Paper

KLF6 inhibited oral cancer migration and invasion via downregulation of mesenchymal markers and inhibition of MMP-9 activities

Li-Sung Hsu1, 2*, Ren-Hung Huang3*, Hung-Wen Lai4, 5, Hui-Ting Hsu3, 6, 7, 8, Wen-Wei Sung6, 7, 8, 9, Ming-Ju Hsieh10, 11, Chong-Yu Wu1, Yueh-Min Lin3, 8, Mu-Kuan Chen7, 12, Yu-Sheng Lo10, Chih-Jung Chen3, 7, 8✉

1. Institute of Biochemistry, Microbiology, and Immunology, Chung Shan Medical University, Taichung, Taiwan;
2. Clinical Laboratory, Chung Shan Medical University Hospital Taichung, Taiwan;
3. Department of Surgical Pathology, Changhua Christian Hospital, Changhua, Taiwan;
4. Department of Surgery, Changhua Christian Hospital, Changhua, Taiwan;
5. School of Medicine, National Yang Ming University, Taipei, Taiwan;
6. Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan;
7. School of Medicine, Chung Shan Medical University, Taichuang, Taiwan;
8. Department of Medical Technology, Jen-Teh Junior College of Medicine, Nursing and Management, Miaoli, Taiwan;
9. Department of Medical Education, Chung Shan Medical University Hospital, Taichung, Taiwan;
10. Cancer Research Center, Changhua Christian Hospital, Changhua, Taiwan;
11. Graduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan;
12. Department of Otorhinolaryngology, Head and Neck Surgery, Changhua Christian Hospital, Changhua, Taiwan.
* These authors contributed equally.

Abstract

Krüppel-like factors can bind to specific DNA motifs and regulate various cellular functions, such as metabolism, cell proliferation, and differentiation. Krüppel-like factor 6 (KLF6), a member of this family, is downregulated in human cancers. Oral cancer is a highly prevalent type in Taiwan. Although KLF6 overexpression in human cancer cells inhibits cell proliferation, induces apoptosis, and attenuates cell migration, the effects of KLF6 on oral cancer remains poorly elucidated. This study investigated the role of KLF6 in oral cancer tumorigenesis. Immunohistochemical staining revealed that nuclear KLF6 level was significantly and inversely associated with tumor size and stages. KLF6 overexpression attenuated the migration and invasion of oral cancer SAS cells. Zymography assay demonstrated that KLF6 inhibited the activities of matrix metalloproteinase 9 (MMP-9) and weakened the expression of mesenchymal markers, such as snail, slug, and vimentin. Our study is the first to provide demonstrate that KLF6 functions as a tumor suppressor gene and prevents the metastasis of oral cancer cells.

Keywords: kruppel-like factor 6 (KLF6), oral cancer, migration, matrix metalloproteinase 9.

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions.
How to cite this article:
Hsu LS, Huang RH, Lai HW, Hsu HT, Sung WW, Hsieh MJ, Wu CY, Lin YM, Chen MK, Lo YS, Chen CJ. KLF6 inhibited oral cancer migration and invasion via downregulation of mesenchymal markers and inhibition of MMP-9 activities. Int J Med Sci 2017; 14(6):530-535. doi:10.7150/ijms.19024. Available from http://www.medsci.org/v14p0530.htm