Int J Med Sci 2017; 14(3):294-301. doi:10.7150/ijms.18137
High quality in vitro expansion of human endothelial progenitor cells of human umbilical vein origin
1. Key Laboratory of Pathobiology, Ministry of Education, Norman Bethune College of Medicine, Jilin University, Changchun, China;
2. The Second Hospital of Jilin University, Changchun, China;
3. The First Hospital of Jilin University, Changchun, China.
The limited availability of qualified endothelial progenitor cells (EPCs) is a major challenge for regenerative medicine. In the present study, we isolated human EPCs from human umbilical vein endothelial cells (HUVECs) by using magnetic micro-beads coated with an antibody against human CD34. Flow cytometric assay showed that majority of these cells expressed VEGFR2 (KDR), CD34 and CD133, three molecular markers for early EPCs. It was also found that a bioreactor micro-carrier cell culture system (bio-MCCS) was superior to dish culture for in vitro expansion of EPCs. It expanded more EPCs which were in the early stage, as shown by the expression of characteristic molecular markers and had better angiogenic potential, as shown by matrix-gel based in vitro angiogenesis assay. These results suggest that HUVECs might be a novel promising resource of EPCs for regenerative medicine and that a bio-MCCS cell culture system might be broadly used for in vitro expansion of EPCs.
Keywords: endothelial progenitor cells, micro-carrier, angiogenesis, cell therapy.
Mou Y, Yue Z, Zhang H, Shi X, Zhang M, Chang X, Gao H, Li R, Wang Z. High quality in vitro expansion of human endothelial progenitor cells of human umbilical vein origin. Int J Med Sci 2017; 14(3):294-301. doi:10.7150/ijms.18137. Available from http://www.medsci.org/v14p0294.htm