International Journal of Medical Sciences

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24 October 2017

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Int J Med Sci 2016; 13(9):680-685. doi:10.7150/ijms.16267

Research Paper

Suppression of Myostatin Stimulates Regenerative Potential of Injured Antigravitational Soleus Muscle in Mice under Unloading Condition

Yoshitaka Ohno1, Yusuke Matsuba2, Naohiro Hashimoto3, Takao Sugiura4, Yoshinobu Ohira5, Toshitada Yoshioka6, Katsumasa Goto1,7 ✉

1. Laboratory of Physiology, School of Health Sciences, Toyohashi SOZO University, Toyohashi, Aichi, Japan;
2. Department of Orthopaedic Surgery, St. Marianna University School of Medicine, Kawasaki, Kanagawa, Japan;
3. Department of Regenerative Medicine, National Center for Geriatrics and Gerontology, Obu, Aichi, Japan;
4. Faculty of Education, Yamaguchi University, Yamaguchi City, Yamaguchi, Japan;
5. Graduate School of Health and Sports Science, Doshisha University, Kyotanabe, Kyoto, Japan;
6. Hirosaki Gakuin University, Hirosaki, Aomori, Japan;
7. Department of Physiology, Graduate School of Health Sciences, Toyohashi SOZO University, Toyohashi, Aichi, Japan.

Abstract

Effects of myostatin (MSTN)-suppression on the regeneration of injured skeletal muscle under unloading condition were investigated by using transgenic mice expressing a dominant-negative form of MSTN (MSTN-DN). Both MSTN-DN and wild-type (WT) mice were subjected to continuous hindlimb suspension (HS) for 6 weeks. Cardiotoxin (CTX) was injected into left soleus muscle under anesthesia 2 weeks after the initiation of HS. Then, the soleus muscles were excised following 6-week HS (4 weeks after CTX-injection). CTX-injection caused to reduce the soleus fiber cross-sectional area (CSA) in WT mice under both unloading and weight-bearing conditions, but not in MSTN-DN mice. Under unloading condition, CTX-injected muscle weight and fiber CSA in MSTN-DN mice were significantly higher than those in WT mice. CTX-injected muscle had many damaged and regenerating fibers having central nuclei in both WT and MSTN-DN mice. Significant increase in the population of Pax7-positive nuclei in CTX-injected muscle was observed in MSTN-DN mice, but not in WT mice. Evidences indicate that the suppression of MSTN cause to increase the regenerative potential of injured soleus muscle via the increase in the population of muscle satellite cells regardless of unloading conditions.

Keywords: skeletal muscle, muscle regeneration, unloading, myostatin, muscle satellite cell.

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How to cite this article:
Ohno Y, Matsuba Y, Hashimoto N, Sugiura T, Ohira Y, Yoshioka T, Goto K. Suppression of Myostatin Stimulates Regenerative Potential of Injured Antigravitational Soleus Muscle in Mice under Unloading Condition. Int J Med Sci 2016; 13(9):680-685. doi:10.7150/ijms.16267. Available from http://www.medsci.org/v13p0680.htm