International Journal of Medical Sciences

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23 October 2017

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Int J Med Sci 2016; 13(7):500-506. doi:10.7150/ijms.15671

Research Paper

Migration and Differentiation of GFP-transplanted Bone Marrow-derived Cells into Experimentally Induced Periodontal Polyp in Mice

Saeka Matsuda1,2, Masahito Shoumura2, Naoto Osuga2, Hidetsugu Tsujigiwa3, Keisuke Nakano4,5, Norimasa Okafuji1, Takanaga Ochiai1,5, Hiromasa Hasegawa1,5, Toshiyuki Kawakami1✉

1. Department of Hard Tissue Research, Matsumoto Dental University Graduate School of Oral Medicine, Shiojiri, Japan
2. Department of Pediatric Dentistry, Matsumoto Dental University School of Dentistry, Shiojiri, Japan
3. Department of Life Science, Faculty of Science, Okayama University of Science, Okayama, Japan
4. Department of Oral Pathology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan
5. Department of Oral Pathology, Matsumoto Dental University School of Dentistry, Shiojiri, Japan

Abstract

Perforation of floor of the dental pulp is often encountered during root canal treatment in routine clinical practice of dental caries. If perforation were large, granulation tissue would grow to form periodontal polyp. Granulation tissue consists of proliferating cells however their origin is not clear. It was shown that the cells in granulation tissue are mainly from migration of undifferentiated mesenchymal cells of the bone marrow. Hence, this study utilized GFP bone marrow transplantation mouse model. The floor of the pulp chamber in maxillary first molar was perforated using ½ dental round bur. Morphological assessment was carried out by micro CT and microscopy and GFP cell mechanism was further assessed by immunohistochemistry using double fluorescent staining with GFP-S100A4; GFP-Runx2 and GFP-CD31. Results of micro CT revealed alveolar bone resorption and widening of periodontal ligament. Histopathological examination showed proliferation of fibroblasts with some round cells and blood vessels in the granulation tissue. At 2 weeks, the outermost layer of the granulation tissue was lined by squamous cells with distinct intercellular bridges. At 4 weeks, the granulation tissue became larger than the perforation and the outermost layer was lined by relatively typical stratified squamous epithelium. Double immunofluorescent staining of GFP and Runx2 revealed that both proteins were expressed in spindle-shaped cells. Double immunofluorescent staining of GFP and CD31 revealed that both proteins were expressed in vascular endothelial cells in morphologically distinct vessels. The results suggest that fibroblasts, periodontal ligament fibroblasts and blood vessels in granulation tissue were derived from transplanted-bone marrow cells. Thus, essential growth of granulation tissue in periodontal polyp was caused by the migration of undifferentiated mesenchymal cells derived from bone marrow, which differentiated into fibroblasts and later on differentiated into other cells in response to injury.

Keywords: Periodontal polyp, Periodontal ligament, Cell differentiation, Immunohistochemistry

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How to cite this article:
Matsuda S, Shoumura M, Osuga N, Tsujigiwa H, Nakano K, Okafuji N, Ochiai T, Hasegawa H, Kawakami T. Migration and Differentiation of GFP-transplanted Bone Marrow-derived Cells into Experimentally Induced Periodontal Polyp in Mice. Int J Med Sci 2016; 13(7):500-506. doi:10.7150/ijms.15671. Available from http://www.medsci.org/v13p0500.htm