International Journal of Medical Sciences

Impact factor
2.399

21 October 2017

ISSN 1449-1907 News feeds of published articles

My Manuscript | My Account

Journal of Genomics now in PubMed/PubMed Central. Submit manuscript...

Journal of Biomedicinenew

Theranostics

Journal of Cancer

Oncomedicine

International Journal of Biological Sciences

Journal of Genomics

Journal of Bone and Joint Infection (JBJI)

Nanotheranostics

PubMed Central Indexed in Journal Impact Factor

Int J Med Sci 2015; 12(7):544-551. doi:10.7150/ijms.12217

Research Paper

Cytological Kinetics of Periodontal Ligament in an Experimental Occlusal Trauma Model

Tatsuo Takaya1✉, Hiroaki Mimura1, Saeka Matsuda2, Keisuke Nakano2, Hidetsugu Tsujigiwa3, Mihoko Tomida4, Norimasa Okafuji2, Takeo Fujii1, Toshiyuki Kawakami2

1. Department of Oral Health Promotion, Matsumoto Dental University Graduate School of Oral Medicine, Shiojiri, Japan
2. Department of Hard Tissue Research, Matsumoto Dental University Graduate School of Oral Medicine, Shiojiri, Japan
3. Department of Life Science, Faculty of Science, Okayama University of Science, Okayama, Japan
4. Department of Oral and Maxillofacial Biology, Matsumoto Dental University School of Dentistry, Shiojiri, Japan

Abstract

Using a model of experimental occlusal trauma in mice, we investigated cytological kinetics of periodontal ligament by means of histopathological, immunohistochemical, and photographical analysis methods. Periodontal ligament cells at furcation areas of molar teeth in the experimental group on day 4 showed a proliferation tendency of periodontal ligament cells. The cells with a round-shaped nucleus deeply stained the hematoxylin and increased within the day 4 specimens. Ki67 positive nuclei showed a prominent increase in the group on days 4 and 7. Green Fluorescent Protein (GFP) positivity also revealed cell movement but was slightly slow compared to Ki67. It indicated that restoration of mechanism seemed conspicuous by osteoclasts and macrophages from bone-marrow-derived cells for the periodontal ligament at the furcation area. It was suggested that the remodeling of periodontal ligament with cell acceleration was evoked from the experiment for the group on day 4 and after day 7. Periodontal ligament at the furcation area of the molar teeth in this experimental model recovered using the cells in situ and the bone-marrow-derived cells.

Keywords: Occlusal trauma, Periodontal tissue, Green fluorescent protein (GFP), Ki67, Mouse

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) License. See http://ivyspring.com/terms for full terms and conditions.
How to cite this article:
Takaya T, Mimura H, Matsuda S, Nakano K, Tsujigiwa H, Tomida M, Okafuji N, Fujii T, Kawakami T. Cytological Kinetics of Periodontal Ligament in an Experimental Occlusal Trauma Model. Int J Med Sci 2015; 12(7):544-551. doi:10.7150/ijms.12217. Available from http://www.medsci.org/v12p0544.htm