Inhibition of HCV 5′-NTR and Core Expression by a Small Hairpin RNA Delivered by a Histone Gene Carrier, HPhA

Ding, Yanhua; Zhang, Hong; Li, Yuxiang; Wu, Di; He, Shumei; Wang, Yang; Li, Yuanyuan; Wang, Feng; Niu, Junqi

doi:10.7150/ijms.5632

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Int J Med Sci 2013; 10(8):957-964. doi:10.7150/ijms.5632 This issue Cite

Research Paper

Inhibition of HCV 5′-NTR and Core Expression by a Small Hairpin RNA Delivered by a Histone Gene Carrier, HPhA

Yanhua Ding¹, Hong Zhang¹, Yuxiang Li², Di Wu³, Shumei He², Yang Wang², Yuanyuan Li⁴, Feng Wang², Junqi Niu^{2 ✉}

1. Phase I Clinical Trial Unit, First Hospital, Jilin University, Changchun 130021, China;
2. First Hospital, Jilin University, Changchun, China;
3. Cancer Hospital of Jilin Province, Changchun, China;
4. Key Laboratory for Molecular and Engineering of Ministry of Education, Jilin University, Changchun, China.

Citation:

Ding Y, Zhang H, Li Y, Wu D, He S, Wang Y, Li Y, Wang F, Niu J. Inhibition of HCV 5′-NTR and Core Expression by a Small Hairpin RNA Delivered by a Histone Gene Carrier, HPhA. Int J Med Sci 2013; 10(8):957-964. doi:10.7150/ijms.5632. https://www.medsci.org/v10p0957.htm

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Abstract

siRNA (small interfering RNA) interference represents an exciting new technology that could have therapeutic applications for the treatment of viral infections. However, a major challenge in the use of siRNA as a therapeutic agent is the development of a suitable delivery system. We demonstrated that a new non-viral transgene carrier, recombinant archaeal histone from the hyperthermophile Pyrococcus horikoshii OT3 (HPhA), can transfect short hairpin RNA (shRNA) expressing plasmids into HL-7702 cells to inhibit the expression of HCV 5'NTR and Core protein and mRNA. Plasmids Psilencirle transfected by HPhA inhibited the expression of HCV 5'-NTR and Core protein and mRNA in HL-7702 cells. The transfection efficiency of HPhA in HL-7702 cells was not affected by 10% fetal calf serum (FCS). HPhA exhibited effects of transfection without apparent toxicity, and with high affinity for DNA. This suggests that HPhA may be useful for RNAi-based gene therapy in vivo.

Keywords: RNA interference, hepatitis C virus, small hairpin RNA, gene therapy, HPhA

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APA

Ding, Y., Zhang, H., Li, Y., Wu, D., He, S., Wang, Y., Li, Y., Wang, F., Niu, J. (2013). Inhibition of HCV 5′-NTR and Core Expression by a Small Hairpin RNA Delivered by a Histone Gene Carrier, HPhA. International Journal of Medical Sciences, 10(8), 957-964. https://doi.org/10.7150/ijms.5632.

ACS

Ding, Y.; Zhang, H.; Li, Y.; Wu, D.; He, S.; Wang, Y.; Li, Y.; Wang, F.; Niu, J. Inhibition of HCV 5′-NTR and Core Expression by a Small Hairpin RNA Delivered by a Histone Gene Carrier, HPhA. Int. J. Med. Sci. 2013, 10 (8), 957-964. DOI: 10.7150/ijms.5632.

NLM

CSE

Ding Y, Zhang H, Li Y, Wu D, He S, Wang Y, Li Y, Wang F, Niu J. 2013. Inhibition of HCV 5′-NTR and Core Expression by a Small Hairpin RNA Delivered by a Histone Gene Carrier, HPhA. Int J Med Sci. 10(8):957-964.

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