Int J Med Sci 2013; 10(4):385-391. doi:10.7150/ijms.5182
Simplified Stereological Evaluation of Renal Morphology after Unilateral Ureteral Obstruction
1. Department of Pediatrics, the First Affiliated Hospital of Anhui Medical University, No. 218 Ji-Xi Road, Hefei 230022, PR China;
2. Department of Urology, Anhui Provincial Hospital, Anhui Medical University, No. 17 Lu-Jiang Road, Hefei 230001, PR China;
3. Department of Pediatrics, the First Affiliated Hospital of Guangxi Medical University, No. 6 Shuang-Yong Road, Nanning 530021, PR China.
Objective and methods: This study established a simple stereological method to obtain quantitative information about two- or three-dimensional structures based on observations from kidney sections in the unilateral ureteral obstruction(UUO) model. Results: Tubulointerstitial area(TA) and TA/the area of a rectangular field(RA) were raised gradually, but significantly, in the obstructed kidney from 1 to 3months post-ligation in comparison to the sham kidney of sham-operated rats(SOR). On the contrary, glomerular area(GA) and glomerular volume(GV) were decreased progressively over time, but significantly, in the obstructed kidney from 3weeks to 3months post-ligation compared to the sham kidney of SOR. UUO caused a progressive decline of TA and TA/RA in the contralateral kidney. More specifically, there were significant decreases in TA at 1,2,3months post-ligation, while in TA/RA only at 3months post-ligation in comparison to the right kidney of SOR. In contrast, GA and GV enhanced in a time-dependent manner in the contralateral kidney, in which the difference in GA reached significance only at 3months post-ligation, whereas the difference in GV reached significance from 1 to 3months post-ligation when comparing with the right kidney of SOR. Conclusions: Our results confirmed two typical features of obstructive nephropathy, including widen interstitial space and glomerular atrophy in the obstructed kidney, and compensatory growth of the contralateral kidney.
Keywords: Compensatory hypertrophy, kidney section, stereological method, unilateral ureteral obstruction, Wistar rat.
Hu P, Zhao XQ, Wang J, Cai J, Hu B, Lu L, Xuan Q, Qin YH. Simplified Stereological Evaluation of Renal Morphology after Unilateral Ureteral Obstruction. Int J Med Sci 2013; 10(4):385-391. doi:10.7150/ijms.5182. Available from http://www.medsci.org/v10p0385.htm