21 January 2018
Int J Med Sci 2013; 10(3):286-291. doi:10.7150/ijms.5343
Emulsified Isoflurane Preconditioning Protects Isolated Rat Kupffer Cells against Hypoxia/Reoxygenation-Induced Injury
1. Department of Anaesthesia and Intensive Care, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai, 200438, China.
Objective: To investigate the protective effect of emulsified isoflurane (EI) preconditioning on isolated rat Kupffer cells (KCs) subjected to hypoxia/reoxygenation (H/R)-induced injury.
Materials and methods: KCs were isolated by collagenase digestion and purified by Percoll density gradient centrifugation. Primary cultured KCs were divided into five groups: control, H/R plus 0.1% lipid preconditioning, and H/R plus 0.05%, 0.1% or 0.2% emulsified isoflurane preconditioning groups. H/R was induced by 4 h of hypoxia followed by 6 h of reoxygenation. Reactive oxygen species (ROS) production in the KCs and the concentration of tumor necrosis factor-α (TNF-α) in the KC culture media were measured, and the apoptosis of KCs was assayed concomitantly.
Results: ROS and TNF-α production were markedly induced in the H/R + lipid group, and lower in the 0.2% and 0.1% EI groups (P<0.05). The apoptotic rate in the H/R + lipid group was significantly higher than that in the 0.2% and 0.1% EI groups (P<0.05).
Conclusions: Emulsified isoflurane protects isolated rat KCs against H/R induced injury by decreasing the production of ROS and TNF-α and attenuating apoptosis in KCs.
Keywords: kupffer cells, emulsified isoflurane, oxidative stress, rats, apoptosis.
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How to cite this article:
Wang Z, Lv H, Song S, Shen X, Yang L, Yu W. Emulsified Isoflurane Preconditioning Protects Isolated Rat Kupffer Cells against Hypoxia/Reoxygenation-Induced Injury. Int J Med Sci 2013; 10(3):286-291. doi:10.7150/ijms.5343. Available from http://www.medsci.org/v10p0286.htm