Int J Med Sci 2013; 10(3):235-242. doi:10.7150/ijms.5524

Research Paper

An Association Study on Genetic Polymorphisms of Rab37 Gene with the Risk of Esophageal Squamous Cell Carcinoma in a Chinese Han Population

Xiaofeng Xu1, Xingying Guan1, Huansheng Tao1, Kang Yang2✉, Yun Bai1✉

1. Department of Medical Genetics, Third Military Medical University, Chongqing 400038, People's Republic of China;
2. Department of Thoracic and Cardiac Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, People's Republic of China.

Abstract

Background: Rab37 encodes a Rab GTPase which regulates the vesicular transport of exocytosis. But the different findings in two types of cancers made its roles in oncology more confused. In this study, a clinical research on genetic polymorphisms was performed to evaluate the association between Rab37 and esophageal squamous cell carcinoma (ESCC).

Methods: The mRNA expression was tested by reverse transcription-polymerase chain reaction (RT-PCR) in four ESCC cell lines. A case-control study including 212 ESCC patients and 213 cancer-free controls was genotyped by PCR-restriction fragment length polymorphism. The Hardy-Weinberg equilibrium test, association analysis and haplotype analysis were performed with SPSS and SHEsis software respectively.

Results: Rab37 mRNA could be specifically detected in two ESCC cell lines, EC109 and EC9706, but not in KYS150 and KYS450. The allele, genotype and haplotype frequencies of rs9904078G>A, rs2034310T>C and rs5018106T>C, located in Rab37, did not significantly differ between the patients and the controls. No association between the polymorphisms and the TNM stages of patients was found.

Conclusions: Rab37 mRNA was specifically expressed in some ESCC cell lines but its genetic polymorphisms were not associated with ESCC.

Keywords: Rab37, Esophageal squamous cell carcinoma, Polymorphisms, Case-control study.

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How to cite this article:
Xu X, Guan X, Tao H, Yang K, Bai Y. An Association Study on Genetic Polymorphisms of Rab37 Gene with the Risk of Esophageal Squamous Cell Carcinoma in a Chinese Han Population. Int J Med Sci 2013; 10(3):235-242. doi:10.7150/ijms.5524. Available from http://www.medsci.org/v10p0235.htm