International Journal of Medical Sciences

Impact factor
2.399

19 October 2017

ISSN 1449-1907 News feeds of published articles

My Manuscript | My Account

Journal of Genomics now in PubMed/PubMed Central. Submit manuscript...

Journal of Biomedicinenew

Theranostics

Journal of Cancer

Oncomedicine

International Journal of Biological Sciences

Journal of Genomics

Journal of Bone and Joint Infection (JBJI)

Nanotheranostics

PubMed Central Indexed in Journal Impact Factor

Int J Med Sci 2013; 10(3):230-234. doi:10.7150/ijms.5278

Research Paper

Detection of Hypermethylated Spastic Paraplegia-20 in Stool Samples of Patients with Colorectal Cancer

Hao Zhang, Yong-Chun Song, Cheng-Xue Dang

Department of Surgical Oncology the First Affiliated Hospital, Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi 710061, China.

Abstract

Background. Analysis of aberrant hypermethylation in stool DNA might provide a novel strategy for noninvasive detection of colorectal cancer.

Aims. To explore the feasibility of detecting hypermethylation in Spastic paraplegia-20 promoter as a stool-based DNA marker for detection of colorectal cancer.

Methods. We collected 96 tissue and stool samples from patients with colorectal cancer and 30 stool samples healthy individuals.

Results. Hypermethylated Spastic paraplegia-20 occurs in 85.4% (82/96) of patients with colorectal cancer in the tissue samples. In the stool samples, the results indicate 80.2% (77/96) sensitivity and 100% (30/30) specificity of the test for detecting colorectal cancer by using the stool samples as a noninvasive method.

Conclusions. The study reveals that hypermethylation in Spastic paraplegia-20 promoter is a highly specific and sensitive biomarker for screening colorectal cancer in stool samples as a noninvasive method.

Keywords: Colorectal cancer, Screening, SPG20, Stool DNA, Hypermethylation, Biomarker.

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) License. See http://ivyspring.com/terms for full terms and conditions.
How to cite this article:
Zhang H, Song YC, Dang CX. Detection of Hypermethylated Spastic Paraplegia-20 in Stool Samples of Patients with Colorectal Cancer. Int J Med Sci 2013; 10(3):230-234. doi:10.7150/ijms.5278. Available from http://www.medsci.org/v10p0230.htm