International Journal of Medical Sciences

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14 December 2017

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Int J Med Sci 2013; 10(1):68-72. doi:10.7150/ijms.5256

Short Research Communication

Long Term and Standard Incubations of WST-1 Reagent Reflect the Same Inhibitory Trend of Cell Viability in Rat Airway Smooth Muscle Cells

Lei-Miao Yin, Yin Wei, Yu Wang, Yu-Dong Xu, Yong-Qing Yang

Laboratory of Molecular Biology, Shanghai Research Institute of Acupuncture and Meridian, Yue Yang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200030, China.

Abstract

The WST-1 assay is an efficient test for cell viability measurement and the standard incubation time is 2h. In order to test if one-time addition of WST-1 reagent can reflect the relative cell viability trend of the testing agents at different time points, the effects of 2h standard incubation time and long term incubation time (2h+24h, 2h+48h) of WST-1 were compared in the rat airway smooth muscle cells (ASM cells) after adding of the testing protein MRP-14. Our study demonstrated that the effect of different dosages of the protein after 2h WST-1 incubation on ASM cells showed a tendency of inhibition and achieved the maximal inhibition effect at 72h. The relative cell viability trend of the 2h+24h group was the same to that of the 2h WST-1 incubation, which means that 24h prolonged incubation time of WST-1 reagent could still reflect the relative cell viability trend. In conclusion, the study suggested that the WST-1 is a proper candidate reagent for continuous monitation of cell viability.

Keywords: WST-1, Cell viability, Rat airway smooth muscle cells.

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How to cite this article:
Yin LM, Wei Y, Wang Y, Xu YD, Yang YQ. Long Term and Standard Incubations of WST-1 Reagent Reflect the Same Inhibitory Trend of Cell Viability in Rat Airway Smooth Muscle Cells. Int J Med Sci 2013; 10(1):68-72. doi:10.7150/ijms.5256. Available from http://www.medsci.org/v10p0068.htm