Int J Med Sci 2012; 9(10):862-871. doi:10.7150/ijms.5027

Research Paper

Bone Morphogenetic Protein-9 Induces Osteogenic Differentiation of Rat Dental Follicle Stem Cells in P38 and ERK1/2 MAPK Dependent Manner

Conghua Li1, Xia Yang1✉, Yujuan He2, Guo Ye1, Xiaodong Li1, Xiaonan Zhang1, Lan Zhou2, Feng Deng1✉

1. Department of Stomatology, Research Center for Oral Diseases and Biomedicine in Chongqing, Affiliated Stomatological Hospital, Chongqing Medical University, Chongqing 400015, China;
2. Key Laboratory of Clinical Laboratory Science of the Ministry of Education, Chongqing Medical University, China.

Abstract

Dental follicle stem cells are a group of cells possessing osteogenic, adipogenetic and neurogenic differentiations, but the specific mechanism underlying the multilineage differentiation remains still unclear. Great attention has been paid to bone morphogenetic protein-9 (BMP-9) due to its potent osteogenic activity. In the present study, rat dental follicle stem cells were isolated and purified, and cells of passage 3 underwent adenovirus mediated BMP-9 gene transfection to prepare dental follicle stem cells with stable BMP-9 expression. Detection of alkaline phosphatase (ALP) and calcium deposition showed dental follicle stem cells transfected with BMP-9 gene could significantly promote the osteogenesis. In addition, SB203580 and PD98059 were employed to block the p38 mitogen-activated protein kinase (p38MAPK) and extracellular signal-regulated kinase (ERK1/2), respectively. Detection of ALP and calcium deposition revealed the BMP-9 induced osteogenic differentiation of dental follicle stem cells depended on MAPK signaling pathway.

Keywords: Dental follicle stem cells, rat recombinant adenovirus transfection, bone morphogenetic protein-9, osteogenic differentiation, signaling pathway.

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How to cite this article:
Li C, Yang X, He Y, Ye G, Li X, Zhang X, Zhou L, Deng F. Bone Morphogenetic Protein-9 Induces Osteogenic Differentiation of Rat Dental Follicle Stem Cells in P38 and ERK1/2 MAPK Dependent Manner. Int J Med Sci 2012; 9(10):862-871. doi:10.7150/ijms.5027. Available from http://www.medsci.org/v09p0862.htm