20 March 2018
Int J Med Sci 2012; 9(5):339-352. doi:10.7150/ijms.4414
BioShuttle Mobility in Living Cells Studied with High-Resolution FCS & CLSM Methodologies
1. German Cancer Research Center, Dept. of Imaging and Radiooncology, INF 280, D-69120 Heidelberg, Germany.
With the increase in molecular diagnostics and patient-specific therapeutic approaches, the delivery and targeting of imaging molecules and pharmacologically active agents gain increasing importance. The ideal delivery system does not exist yet. The realization of two features is indispensable: first, a locally high concentration of target-specific diagnostic and therapeutic molecules; second, the broad development of effective and safe carrier systems. Here we characterize the transport properties of the peptide-based BioShuttle transporter using FFM and CLSM methods. The modular design of BioShuttle-based formulations results in a multi-faceted field of applications, also as a theranostic tool.
Keywords: Drug Delivery, BioShuttle, Confocal Laser Scanning Microscopy-CLSM, Fluorescence Correlation Spectroscopy-FCS, Fluorescence Fluctuation Microscopy-FFM, Ligation chemistry, Non-Viral Carrier Systems, Spinning Disc Microscopy-SDM.
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How to cite this article:
Braun K, Beining M, Wiessler M, Lammers T, Pipkorn R, Hennrich U, Nokihara K, Semmler W, Debus J, Waldeck W. BioShuttle Mobility in Living Cells Studied with High-Resolution FCS & CLSM Methodologies. Int J Med Sci 2012; 9(5):339-352. doi:10.7150/ijms.4414. Available from http://www.medsci.org/v09p0339.htm