22 March 2018
Int J Med Sci 2011; 8(5):428-432. doi:10.7150/ijms.8.428
A Comparison of Immuncapture Agglutination and ELISA Methods in Serological Diagnosis of Brucellosis
1. Department of Medical Microbiology, Selcuk University Meram Faculty of Medicine, Konya, Turkey
Background: Different serological tests are used in serologic diagnosis of brucellosis. The most widely used of these are Standard Tube Agglutination and Coombs anti-brucella tests. Whereas ELISA Ig M and Ig G tests have been in use for a long time, immuncapture agglutination test has been recently introduced and used in serological diagnosis. The aim of this study was to compare diagnostic values of ELISA Ig M and Ig G and immuncapture agglutination tests with Coombs anti-brucella test.
Methods: Sera from 200 patients with presumptive diagnosis of brucellosis were included into the study. Coombs anti-brucella test, ELISA Ig M and Ig G tests and Immuncapture test were investigated in these sera. Then, sensitivity, specificity, negative predictive and positive predictive values were calculated.
Results: Sensitivity, specificity, negative predictive and positive predictive values were found to be 90,6 %, 76,3 %, 94,2 %, and 65,9 % respectively for the Immuncapture test, whereas they were found to be 73,7 %, 58,9 %, 84,2 %, and 42,8 % for Ig G and 72,2 %, 67,8 %, 85,2 %, and 48,7 % for Ig M. The Immuncapture test was found to be compatible with ELISA Ig M and Ig G tests but it was statistically incompatible with Coombs anti-brucella test.
Conclusions: Immuncapture agglutination test yields similar results to those of Coombs anti-brucella test. This test is a useful test by virtue of the fact that it determines blocking antibodies in the diagnosis and follow-up of brucellosis.
Keywords: Brucellosis, immuncapture, ELISA, IgG, IgM, serologic diagnosis
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How to cite this article:
Özdemir M, Feyzioğlu B, Kurtoğlu MG, Doğan M, Dağı HT, Yüksekkaya Ş, Keşli R, Baysal B. A Comparison of Immuncapture Agglutination and ELISA Methods in Serological Diagnosis of Brucellosis. Int J Med Sci 2011; 8(5):428-432. doi:10.7150/ijms.8.428. Available from http://www.medsci.org/v08p0428.htm