A Comparison of Immuncapture Agglutination and ELISA Methods in Serological Diagnosis of Brucellosis

Özdemir, Mehmet; Feyzioğlu, Bahadır; Kurtoğlu, Muhammed Güzel; Doğan, Metin; Dağı, Hatice Türk; Yüksekkaya, Şerife; Keşli, Recep; Baysal, Bülent

doi:10.7150/ijms.8.428



Theranostics

International Journal of Biological Sciences

Nanotheranostics

Journal of Cancer

Journal of Genomics

Global reach, higher impact

Full Text | PDF

Int J Med Sci 2011; 8(5):428-432. doi:10.7150/ijms.8.428 This issue Cite

Research Paper

A Comparison of Immuncapture Agglutination and ELISA Methods in Serological Diagnosis of Brucellosis

Mehmet Özdemir^1✉, Bahadır Feyzioğlu², Muhammed Güzel Kurtoğlu⁴, Metin Doğan², Hatice Türk Dağı³, Şerife Yüksekkaya⁴, Recep Keşli⁴, Bülent Baysal¹

1. Department of Medical Microbiology, Selcuk University Meram Faculty of Medicine, Konya, Turkey
2. Medical Microbiology Laboratory, Karaman State Hospital, Karaman, Turkey
3. Medical Microbiology Laboratory, Batman State Hospital, Batman,Turkey
4. Medical Microbiology Laboratory, Konya Education and Research Hospital, Konya, Turkey

Citation:

Özdemir M, Feyzioğlu B, Kurtoğlu MG, Doğan M, Dağı HT, Yüksekkaya Ş, Keşli R, Baysal B. A Comparison of Immuncapture Agglutination and ELISA Methods in Serological Diagnosis of Brucellosis. Int J Med Sci 2011; 8(5):428-432. doi:10.7150/ijms.8.428. https://www.medsci.org/v08p0428.htm

Other styles

Abstract

Background: Different serological tests are used in serologic diagnosis of brucellosis. The most widely used of these are Standard Tube Agglutination and Coombs anti-brucella tests. Whereas ELISA Ig M and Ig G tests have been in use for a long time, immuncapture agglutination test has been recently introduced and used in serological diagnosis. The aim of this study was to compare diagnostic values of ELISA Ig M and Ig G and immuncapture agglutination tests with Coombs anti-brucella test.

Methods: Sera from 200 patients with presumptive diagnosis of brucellosis were included into the study. Coombs anti-brucella test, ELISA Ig M and Ig G tests and Immuncapture test were investigated in these sera. Then, sensitivity, specificity, negative predictive and positive predictive values were calculated.

Results: Sensitivity, specificity, negative predictive and positive predictive values were found to be 90,6 %, 76,3 %, 94,2 %, and 65,9 % respectively for the Immuncapture test, whereas they were found to be 73,7 %, 58,9 %, 84,2 %, and 42,8 % for Ig G and 72,2 %, 67,8 %, 85,2 %, and 48,7 % for Ig M. The Immuncapture test was found to be compatible with ELISA Ig M and Ig G tests but it was statistically incompatible with Coombs anti-brucella test.

Conclusions: Immuncapture agglutination test yields similar results to those of Coombs anti-brucella test. This test is a useful test by virtue of the fact that it determines blocking antibodies in the diagnosis and follow-up of brucellosis.

Keywords: Brucellosis, immuncapture, ELISA, IgG, IgM, serologic diagnosis

Citation styles

APA

Özdemir, M., Feyzioğlu, B., Kurtoğlu, M.G., Doğan, M., Dağı, H.T., Yüksekkaya, Ş., Keşli, R., Baysal, B. (2011). A Comparison of Immuncapture Agglutination and ELISA Methods in Serological Diagnosis of Brucellosis. International Journal of Medical Sciences, 8(5), 428-432. https://doi.org/10.7150/ijms.8.428.

ACS

Özdemir, M.; Feyzioğlu, B.; Kurtoğlu, M.G.; Doğan, M.; Dağı, H.T.; Yüksekkaya, Ş.; Keşli, R.; Baysal, B. A Comparison of Immuncapture Agglutination and ELISA Methods in Serological Diagnosis of Brucellosis. Int. J. Med. Sci. 2011, 8 (5), 428-432. DOI: 10.7150/ijms.8.428.

NLM

CSE

Özdemir M, Feyzioğlu B, Kurtoğlu MG, Doğan M, Dağı HT, Yüksekkaya Ş, Keşli R, Baysal B. 2011. A Comparison of Immuncapture Agglutination and ELISA Methods in Serological Diagnosis of Brucellosis. Int J Med Sci. 8(5):428-432.

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) License. See http://ivyspring.com/terms for full terms and conditions.