International Journal of Medical Sciences

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23 October 2017

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Int J Med Sci 2011; 8(3):231-238. doi:10.7150/ijms.8.231

Research Paper

Effects of Expanded Human Adipose Tissue-Derived Mesenchymal Stem Cells on the Viability of Cryopreserved Fat Grafts in the Nude Mouse

Myung-Soon Ko1,2, Ji-Youl Jung2, Il-Seob Shin1, Eun-Wha Choi3, Jae-Hoon Kim2, Sung Keun Kang1, Jeong Chan Ra1,✉

1. Stem Cell Research Center, RNL BIO Co., Ltd, Seoul 153-803, Republic of Korea
2. College of Veterinary Medicine, Jeju National University, Jeju 690-756, Republic of Korea
3. Laboratory Animal Research Center, Samsung Biomedical Research Institute, 50 Irwon-dong Gangnam-gu, Seoul 135-710, Republic of Korea

Abstract

Adipose-derived mesenchymal stem cells (AdMSCs) augment the ability to contribute to microvascular remodeling in vivo and to modulate vascular stability in fresh fat grafts. Although cryopreserved adipose tissue is frequently used for soft tissue augmentation, the viability of the fat graft is poor. The effects of culture-expanded human adipose tissue-derived mesenchymal stem cells (hAdMSCs) on the survival and quality of the cryopreserved fat graft were determined. hAdMSCs from the same donor were mixed with fat tissues cryopreserved at -70°C for 8 weeks and injected subcutaneously into 6-week-old BALB/c-nu nude mice. Graft volume and weight were measured, and histology was evaluated 4 and 15 weeks post-transplantation. The hAdMSC-treated group showed significantly enhanced graft volume and weight. The histological evaluation demonstrated significantly better fat cell integrity compared with the vehicle-treated control 4 weeks post-transplantation. No significant difference in graft weight, volume, or histological parameters was found among the groups 15 weeks post-transplantation. The hAdMSCs enhanced the survival and quality of transplanted cryopreserved fat tissues. Cultured and expanded hAdMSCs have reconstructive capacity in cryopreserved fat grafting by increasing the number of stem cells.

Keywords: Human adipose tissue-derived mesenchymal stem cells (hAdMSCs), cryopreserved fat tissues, viability of fat grafts, dose-dependent.

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) License. See http://ivyspring.com/terms for full terms and conditions.
How to cite this article:
Ko MS, Jung JY, Shin IS, Choi EW, Kim JH, Kang SK, Ra JC. Effects of Expanded Human Adipose Tissue-Derived Mesenchymal Stem Cells on the Viability of Cryopreserved Fat Grafts in the Nude Mouse. Int J Med Sci 2011; 8(3):231-238. doi:10.7150/ijms.8.231. Available from http://www.medsci.org/v08p0231.htm