International Journal of Medical Sciences

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Int J Med Sci 2011; 8(2):139-147. doi:10.7150/ijms.8.139

Research Paper

Relationship between Anti-CCP Antibodies and Oxidant and Anti-Oxidant Activity in Patients with Rheumatoid Arthritis

Levent Ediz1✉, Ozcan Hiz1, Halil Ozkol2, Elif Gulcu1, Murat Toprak1, Mehmet Fethi Ceylan3

1. Yuzuncu Yil University, Medical Faculty, Department of Physical Medicine Rehabilitation and Rheumatology, Van, Turkey;
2. Yuzuncu Yil University, Medical Faculty, Department of Medical Biology, Van, Turkey;
3. Yuzuncu Yil University, Medical Faculty, Department of Orthopaedics, Van, Turkey

Abstract

Objective/Aim: A new group of autoantibodies in Rheumatoid Arthritis (RA), the anti-cyclic citrullinated peptide (anti-CCP) antibodies directed to citrulline-containing proteins, which are of value for the severity of RA. Up to date, the relationship between anti-CCP antibodies and oxidant, anti-oxidant activity in patients with RA has not been elucidated in the previous studies. In this study we aimed to investigate the effect of anti-CCP antibodies in the circulation on whole blood, serum and synovial fluid oxidant and anti-oxidant activity in patients with RA. Materials and Methods: RA patients with anti-CCP (+) (n=25) and anti-CCP (-) (n=24) were recruited into the study. All patients had a positive rheumatoid factor (RF). The patients who were under treatment with only non-steroidal antiinflammatory drugs (NSAID) at the study time included in the study. Catalase (CAT), Glutathione peroxidase (GSHPx), Myeloperoxidase (MPO) activities and the levels of Malondialdehyde (MDA) were measured in whole blood, serum and synovial fluid in both groups. Results: There were no significant differences in terms of the mean whole blood and serum antioxidative activity (CAT, GSHpx) and the mean blood and serum MDA and MPO values (oxidative activity), between the patients with anti-CCP(+) and those with anti-CCP(-). There was increased synovial oxidant activity (MDA and MPO levels) (p<0.05) in anti-CCP(+) RA patients with or without ESR negativity when compared with anti-CCP(-) RA patients. There was positive correlation between anti-CCP antibody levels and synovial MDA and MPO levels (r=0.435, p<0.05, r=0.563, p<0.05 respectively) in anti-CCP (+) group. Conclusions: In conclusion, anti-CCP antibody positivity seems to be associated with increased synovial fluid oxidant activity (increased MDA and MPO levels) in patients with RA. These conclusions need to be validated in a larger controlled study population.

Keywords: Oxidative stress, Rheumatoid arthritis, Anti-CCP antibody, Malondialdehyde, Myeloperoxidase, Synovial fluid

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How to cite this article:
Ediz L, Hiz O, Ozkol H, Gulcu E, Toprak M, Ceylan MF. Relationship between Anti-CCP Antibodies and Oxidant and Anti-Oxidant Activity in Patients with Rheumatoid Arthritis. Int J Med Sci 2011; 8(2):139-147. doi:10.7150/ijms.8.139. Available from http://www.medsci.org/v08p0139.htm