International Journal of Medical Sciences

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14 December 2017

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Int J Med Sci 2011; 8(2):114-125. doi:10.7150/ijms.8.114

Research Paper

The Influence of Hyperbaric Oxygen Treatment on the Healing of Experimental Defects Filled with Different Bone Graft Substitutes

Yigit Sirin1✉, Vakur Olgac2, Semra Dogru-Abbasoglu3, Leyla Tapul4, Samil Aktas5, Sinan Soley1

1. Istanbul University, Faculty of Dentistry, Department of Oral Surgery, Istanbul, Turkey
2. Istanbul University, Faculty of Medicine, Department of Oncologic Pathology, Istanbul, Turkey
3. Istanbul University, Faculty of Medicine, Department of Biochemistry, Istanbul, Turkey
4. Istanbul University, Faculty of Medicine, Department of Histology and Embryology, Istanbul, Turkey.
5. Istanbul University, Faculty of Medicine, Department of Undersea and Hyperbaric Medicine, Istanbul, Turkey.

Abstract

To assess potential effects of hyperbaric oxygen (HBOT) on artificial bone grafts, β - Tricalcium phosphate (β-TCP) and calcium phosphate coated bovine bone (CPCBB) substitutes were applied to standard bone defects in rat tibiae. The control defects were left empty. Half of the animals received 60 minutes of 2.4 atmosphere absolute (ATA) of HBOT. Rats were sacrificed at one, two and four weeks. Bone healing was assessed histologically and histomorphometrically using light microscopy. The periosteum over the bone defects was examined ultrastructurally. Cardiac blood was collected to determine the serum osteocalcin levels. The HBOT increased new bone formation in the unfilled controls and β-TCP groups and significantly decreased cartilage matrix and fibrous tissue formations in all groups. Active osteoblasts and highly organized collagen fibrils were prominent in the periosteum of β-TCP and control groups. Serum osteocalcin levels also increased with HBOT. The healing of defects filled with CPCBB was similar to the controls and it did not respond to HBOT. These findings suggested that the HBOT had beneficial effects on the healing of unfilled bone defects and those filled with β-TCP bone substitute but not with CPCBB, indicating a material-specific influence pattern of HBOT.

Keywords: Hyperbaric oxygen, beta tricalcium phosphate, calcium phosphate coated bovine bone, light microscopy, ultrastuctural, rat

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How to cite this article:
Sirin Y, Olgac V, Dogru-Abbasoglu S, Tapul L, Aktas S, Soley S. The Influence of Hyperbaric Oxygen Treatment on the Healing of Experimental Defects Filled with Different Bone Graft Substitutes. Int J Med Sci 2011; 8(2):114-125. doi:10.7150/ijms.8.114. Available from http://www.medsci.org/v08p0114.htm