International Journal of Medical Sciences

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Int J Med Sci 2011; 8(1):56-67. doi:10.7150/ijms.8.56

Research Paper

Helicobacter pylori induces mitochondrial DNA mutation and reactive oxygen species level in AGS cells

Xue-Wen Huang 1,* , Rui-Hua Luo2,*, Qi Zhao 3, Zhong-Ze Shen 4, Li-Li Huang 1, Xian-Yuan An1, Lan-Jing Zhao 1, Jie Wang 5, Yu-Zheng Huang5

1. Department of Clinical Laboratory, Huadong Sanatorium, Wuxi, Jiangsu Province 214065, China
2. Department of Gastroscopy, Huadong Sanatorium, Wuxi, Jiangsu Province 214065, China
3. Department of Clinical Laboratory, People's Hospital, Wuxi, Jiangsu Province 214023, China
4. Jiangsu Internation Travel Healthcare Center, Yangzhou Branch, Yangzhou, Jiangsu Province 225009, China
5. Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu Province 214063, China
* Xue-wen Huang and Rui-hua Luo are co-first authors.

Abstract

To investigate the role of ROS in the helicobacter pylori (Hp) induced mtDNA mutations, AGS cells were treated by extracts of Hp11638 or Hp11638M. The ROS levels, cytochrome C reductions, and intracellular ATP levels were measured. The coding region and the D-Loop region were amplified and sequenced. Results showed the ROS levels, cytochrome C reduction and mtDNA mutations were markedly increased and cell viability decreased after treatment with both Hp extracts, and 616 mutations were detected in D-Loop region and 3 heteroplasmic point mutations in the Cytb gene. No mutations were found in the coding region. The mutation rates of mtDNA D-Loop region were positively correlated with the ROS levels and negatively to the ATP levels.

Keywords: Helicobacter pylori, Reactive Oxygen Species, Mitochondrial DNA, Mutation.

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How to cite this article:
Huang XW, Luo RH, Zhao Q, Shen ZZ, Huang LL, An XY, Zhao LJ, Wang J, Huang YZ. Helicobacter pylori induces mitochondrial DNA mutation and reactive oxygen species level in AGS cells. Int J Med Sci 2011; 8(1):56-67. doi:10.7150/ijms.8.56. Available from http://www.medsci.org/v08p0056.htm