International Journal of Medical Sciences

Impact factor
2.399

18 December 2017

ISSN 1449-1907 News feeds of published articles

My Manuscript | My Account

Journal of Biomedicinenew

Theranostics

Journal of Cancer

Oncomedicine

International Journal of Biological Sciences

Journal of Genomics

Journal of Bone and Joint Infection (JBJI)

Nanotheranostics

PubMed Central Indexed in Journal Impact Factor

Int J Med Sci 2010; 7(3):101-109. doi:10.7150/ijms.7.101

Research Paper

Growth of Microorganisms in Total Parenteral Nutrition Solutions Containing Lipid

Takashi Kuwahara1, Kazuyuki Shimono1, Shinya Kaneda1, Takumi Tamura1, Masao Ichihara2, Yoshifumi Nakashima1

1. Preclinical Assessment Department, Otsuka Pharmaceutical Factory, Inc., Tokushima, Japan.
2. Research and Development Center, Otsuka Pharmaceutical Factory, Inc., Tokushima, Japan.

Abstract

Background: To identify the microorganisms that can grow rapidly in total parenteral nutrition (TPN) solutions, we investigated the growth of the major causes of catheter-related blood stream infection (Staphylococcus aureus, Serratia marcescens, Bacillus cereus, and Candida albicans) in TPN solutions containing lipid. Methods: The pH value of a TPN solution containing lipid (pH 6.0, containing 20 ppm of NaHSO3) was adjusted by the addition of HCl to 5.7, 5.4, or 4.9. The pH value of another TPN solution (pH5.5, containing 400 ppm of NaHSO3) was adjusted by the addition of NaOH to 5.9, 6.3, or 6.8. A specific number of each microorganism was added to 10 mL of each test solution and incubated at room temperature. The number of microorganisms was counted as colony forming units at 0, 24, and 48 hrs later. Results: C albicans increased similarly at any pH values in the TPN solution. The bacterial species also increased rapidly at pH6.0 in the solution containing 20 ppm of NaHSO3, but growth was suppressed as the pH value was reduced, with growth halted at pH4.9. However, these bacterial species did not increase, even at pH5.9, in the other solution containing 400 ppm of NaHSO3. Conclusions: These results suggest that Candida species can grow rapidly in almost all TPN solutions regardless of the acidity, lipid, and NaHSO3; also, some bacterial species may grow in TPN solutions containing lipid unless the pH value is 5.0 or less. Therefore, each TPN solution should be examined whether or not the bacterial species can proliferate.

Keywords: CRBSI, microbial growth, TPN solution, lipid, pH, bisulfite

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) License. See http://ivyspring.com/terms for full terms and conditions.
How to cite this article:
Kuwahara T, Shimono K, Kaneda S, Tamura T, Ichihara M, Nakashima Y. Growth of Microorganisms in Total Parenteral Nutrition Solutions Containing Lipid. Int J Med Sci 2010; 7(3):101-109. doi:10.7150/ijms.7.101. Available from http://www.medsci.org/v07p0101.htm